NGF promotes microglial migration through the activation of its high affinity receptor: Modulation by TGF-β

R. De Simone, E. Ambrosini, D. Carnevale, M. A. Ajmone-Cat, L. Minghetti

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Activation and mobilization of microglia are early events in the majority of brain pathologies. Among the signalling molecules that can affect microglial behaviour, we investigated whether nerve growth factor (NGF) was able to influence microglial motility. We found that NGF induced chemotaxis of microglial cells through the activation of TrkA receptor. In addition, NGF chemotactic activity was increased in the presence of low concentrations (≤ 0.2 ng/ml) of transforming growth factor-β (TGF-β), which at this concentration showed chemotactic activity per se. On the contrary, NGF-induced microglial migration was reduced in the presence of chemokinetic concentration of TGF-β (≥ 2 ng/ml). Finally, both basal and NGF-induced migratory activity of microglial cells was increased after a long-term exposure of primary mixed glial cultures to 2 ng/ml of TGF-β. Our observations suggest that both NGF and TGF-β contribute to microglial recruitment. The chemotactic activities of these two pleiotropic factors could be particularly relevant during chronic diseases in which recruited microglia remove apoptotic neurons in the absence of a typical inflammatory reaction.

Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalJournal of Neuroimmunology
Volume190
Issue number1-2
DOIs
Publication statusPublished - Oct 2007

Fingerprint

Transforming Growth Factors
Nerve Growth Factor
Microglia
trkA Receptor
Early Ambulation
Chemotaxis
Neuroglia
Chronic Disease
Pathology
Neurons
Brain

Keywords

  • Cell migration
  • Inflammation
  • Microglia
  • Neuroprotection

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

Cite this

NGF promotes microglial migration through the activation of its high affinity receptor : Modulation by TGF-β. / De Simone, R.; Ambrosini, E.; Carnevale, D.; Ajmone-Cat, M. A.; Minghetti, L.

In: Journal of Neuroimmunology, Vol. 190, No. 1-2, 10.2007, p. 53-60.

Research output: Contribution to journalArticle

De Simone, R. ; Ambrosini, E. ; Carnevale, D. ; Ajmone-Cat, M. A. ; Minghetti, L. / NGF promotes microglial migration through the activation of its high affinity receptor : Modulation by TGF-β. In: Journal of Neuroimmunology. 2007 ; Vol. 190, No. 1-2. pp. 53-60.
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AU - Minghetti, L.

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