NGF-response of egf- dependent progenitor cells obtained from human sympathetic ganglia

Vincenzo Silani, Gian Domenico Borasio, Feng C. Zhou, Simona Bernasconi, Antonio Pizzuti, Alberto Sampietro, Guglielmo Scarlato

Research output: Contribution to journalArticlepeer-review


SIGNALLING molecules are thought to play a significant role in determining the fate of neural crest progenitor cells. The human sympathetic chain was identified at 6.5, 7.5, 8.2, 10.2 and 11.4 postconception (PC) weeks demonstrating low affinity nerve growth factor (NGF) receptors, and was processed for tissue culture. In the presence of epidermal growth factor (EGF), floating spheres of proliferating progenitor cells were developed in vitro. In the absence of EGF progenitor cells differentiated into tyrosine hydroxylase (TH)-immunoreactive neuronal and TH-negative flat cells. NGF treatment significantly increased neurite outgrowth and survival of TH-immu- noreactive cells. The multipotent cells we isolated differ from previously reported sympathoadrenal progenitors in that they give rise to TH immunoreactive neurones pre-cociously sensitive to NGF.

Original languageEnglish
Pages (from-to)2085-2089
Number of pages5
Issue number16
Publication statusPublished - 1994


  • Epidermal growth factor egf
  • Microtubules
  • Nerve growth factor ngf
  • Neural crest
  • Progenitor cell
  • Sympathetic neurones
  • Tissue culture

ASJC Scopus subject areas

  • Neuroscience(all)


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