Ni2+ enhances Fe2+/peroxide-induced oxidation of arachidonic acid and formation of geno/cytotoxic 4-hydroxynonenal

A possible contributory mechanism in nickel toxicity and allergenicity

Paola Manini, Alessandra Napolitano, Emanuela Camera, Teresa Caserta, Mauro Picardo, Anna Palumbo, Marco D'Ischia

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Ni2+, a toxic, carcinogenic and allergenic agent, affected both the kinetic and chemical courses of the Fe2+-induced oxidation of arachidonic acid (AA) in 0.05 M phosphate buffer (pH 7.4) and at 37°C. At 10 μM concentration, Ni2+ decreased the rate of oxidation of peroxide-free AA (200 μM) promoted by 50 μM Fe2+, as determined by measurement of thiobarbituric acid reactive species (TBARS) and 1H NMR analysis. However, in the presence of low levels of peroxides (e.g. 2%), Ni2+ exerted a significant stimulatory effect on Fe2+-induced AA oxidation and TBARS formation. 1H NMR analysis showed that Ni2+ (10 μM) enhanced formation of genotoxic alkenals including 4-hydroxy-2-nonenal (4-HNE, GC/MS evidence) by Fe2+-promoted degradation of both AA and 15-hydroperoxy-5,8,11,13-eicosatetraenoic acid (15-HPETE) methyl esters. The observed stimulatory effects of Ni2+ on peroxide breakdown and cytotoxic aldehyde formation provide an attractive explanation to the enhanced sensitization capacity of nickel in inflammatory states compared to normal states.

Original languageEnglish
Pages (from-to)9-16
Number of pages8
JournalBBA - General Subjects
Volume1621
Issue number1
DOIs
Publication statusPublished - Apr 7 2003

Fingerprint

Peroxides
Nickel
Arachidonic Acid
Toxicity
Oxidation
Nuclear magnetic resonance
Poisons
Aldehydes
Buffers
Esters
Phosphates
Degradation
Kinetics
4-hydroxy-2-nonenal
thiobarbituric acid
Proton Magnetic Resonance Spectroscopy

Keywords

  • 15-Hydroperoxy-5,8,11,13-eicosatetraenoic acid (15-HPETE)
  • Allergy
  • Lipid peroxidation
  • NMR
  • Thiobarbituric acid reactive species

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Ni2+ enhances Fe2+/peroxide-induced oxidation of arachidonic acid and formation of geno/cytotoxic 4-hydroxynonenal : A possible contributory mechanism in nickel toxicity and allergenicity. / Manini, Paola; Napolitano, Alessandra; Camera, Emanuela; Caserta, Teresa; Picardo, Mauro; Palumbo, Anna; D'Ischia, Marco.

In: BBA - General Subjects, Vol. 1621, No. 1, 07.04.2003, p. 9-16.

Research output: Contribution to journalArticle

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abstract = "Ni2+, a toxic, carcinogenic and allergenic agent, affected both the kinetic and chemical courses of the Fe2+-induced oxidation of arachidonic acid (AA) in 0.05 M phosphate buffer (pH 7.4) and at 37°C. At 10 μM concentration, Ni2+ decreased the rate of oxidation of peroxide-free AA (200 μM) promoted by 50 μM Fe2+, as determined by measurement of thiobarbituric acid reactive species (TBARS) and 1H NMR analysis. However, in the presence of low levels of peroxides (e.g. 2{\%}), Ni2+ exerted a significant stimulatory effect on Fe2+-induced AA oxidation and TBARS formation. 1H NMR analysis showed that Ni2+ (10 μM) enhanced formation of genotoxic alkenals including 4-hydroxy-2-nonenal (4-HNE, GC/MS evidence) by Fe2+-promoted degradation of both AA and 15-hydroperoxy-5,8,11,13-eicosatetraenoic acid (15-HPETE) methyl esters. The observed stimulatory effects of Ni2+ on peroxide breakdown and cytotoxic aldehyde formation provide an attractive explanation to the enhanced sensitization capacity of nickel in inflammatory states compared to normal states.",
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