Nicotinic acetylcholine receptor: A structural model for α-subunit peptide 188-201, the putative binding site for cholinergic agents

C. Gotti; F. Frigerio; M. Bolognesi; R. Longhi; G. Racchetti; F. Clementi

doi:10.1016/0014-5793(88)80598-2

Nicotinic acetylcholine receptor: A structural model for α-subunit peptide 188-201, the putative binding site for cholinergic agents

C. Gotti, F. Frigerio, M. Bolognesi, R. Longhi, G. Racchetti, F. Clementi

Istituti Clinici Scientifici Maugeri Spa – Società Benefit

Research output: Contribution to journal › Article › peer-review

Abstract

A peptide corresponding to amino acid sequence 188-201 of the α-subunit of Torpedo AChR binds α-Bgtx. The S-S bridge between Cys 192 and 193 is essential for the binding as Tyr in position 189. The same sequence 188-201 corresponding to human AChR, which instead of Tyr has a Thr in position 189, binds α-Bgtx with a much lower efficiency. Monoclonal antibodies raised against Torpedo peptide 188-201 recognize Torpedo AChR and antibodies against Torpedo AChR recognize peptide 188-201 indicating that the synthetic peptide and the corresponding sequence in the native molecule share some immunological epitopes. With computer graphics and energy refinement a molecular model of this peptide has been elaborated.

Original language	English
Pages (from-to)	118-122
Number of pages	5
Journal	FEBS Letters
Volume	228
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(88)80598-2
Publication status	Published - Feb 8 1988

Keywords

Computer graphics
Molecular modeling
Monoclonal antibody
Nicotinic acetylcholine receptor
α-Bungarotoxin
α-Toxin binding site

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0014-5793(88)80598-2

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title = "Nicotinic acetylcholine receptor: A structural model for α-subunit peptide 188-201, the putative binding site for cholinergic agents",

abstract = "A peptide corresponding to amino acid sequence 188-201 of the α-subunit of Torpedo AChR binds α-Bgtx. The S-S bridge between Cys 192 and 193 is essential for the binding as Tyr in position 189. The same sequence 188-201 corresponding to human AChR, which instead of Tyr has a Thr in position 189, binds α-Bgtx with a much lower efficiency. Monoclonal antibodies raised against Torpedo peptide 188-201 recognize Torpedo AChR and antibodies against Torpedo AChR recognize peptide 188-201 indicating that the synthetic peptide and the corresponding sequence in the native molecule share some immunological epitopes. With computer graphics and energy refinement a molecular model of this peptide has been elaborated.",

keywords = "Computer graphics, Molecular modeling, Monoclonal antibody, Nicotinic acetylcholine receptor, α-Bungarotoxin, α-Toxin binding site",

author = "C. Gotti and F. Frigerio and M. Bolognesi and R. Longhi and G. Racchetti and F. Clementi",

year = "1988",

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T2 - A structural model for α-subunit peptide 188-201, the putative binding site for cholinergic agents

AU - Gotti, C.

AU - Frigerio, F.

AU - Bolognesi, M.

AU - Longhi, R.

AU - Racchetti, G.

AU - Clementi, F.

PY - 1988/2/8

Y1 - 1988/2/8

N2 - A peptide corresponding to amino acid sequence 188-201 of the α-subunit of Torpedo AChR binds α-Bgtx. The S-S bridge between Cys 192 and 193 is essential for the binding as Tyr in position 189. The same sequence 188-201 corresponding to human AChR, which instead of Tyr has a Thr in position 189, binds α-Bgtx with a much lower efficiency. Monoclonal antibodies raised against Torpedo peptide 188-201 recognize Torpedo AChR and antibodies against Torpedo AChR recognize peptide 188-201 indicating that the synthetic peptide and the corresponding sequence in the native molecule share some immunological epitopes. With computer graphics and energy refinement a molecular model of this peptide has been elaborated.

AB - A peptide corresponding to amino acid sequence 188-201 of the α-subunit of Torpedo AChR binds α-Bgtx. The S-S bridge between Cys 192 and 193 is essential for the binding as Tyr in position 189. The same sequence 188-201 corresponding to human AChR, which instead of Tyr has a Thr in position 189, binds α-Bgtx with a much lower efficiency. Monoclonal antibodies raised against Torpedo peptide 188-201 recognize Torpedo AChR and antibodies against Torpedo AChR recognize peptide 188-201 indicating that the synthetic peptide and the corresponding sequence in the native molecule share some immunological epitopes. With computer graphics and energy refinement a molecular model of this peptide has been elaborated.

KW - Computer graphics

KW - Molecular modeling

KW - Monoclonal antibody

KW - Nicotinic acetylcholine receptor

KW - α-Bungarotoxin

KW - α-Toxin binding site

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Nicotinic acetylcholine receptor: A structural model for α-subunit peptide 188-201, the putative binding site for cholinergic agents

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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