Nicotinic acetylcholine receptor: A structural model for α-subunit peptide 188-201, the putative binding site for cholinergic agents

C. Gotti, F. Frigerio, M. Bolognesi, R. Longhi, G. Racchetti, F. Clementi

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A peptide corresponding to amino acid sequence 188-201 of the α-subunit of Torpedo AChR binds α-Bgtx. The S-S bridge between Cys 192 and 193 is essential for the binding as Tyr in position 189. The same sequence 188-201 corresponding to human AChR, which instead of Tyr has a Thr in position 189, binds α-Bgtx with a much lower efficiency. Monoclonal antibodies raised against Torpedo peptide 188-201 recognize Torpedo AChR and antibodies against Torpedo AChR recognize peptide 188-201 indicating that the synthetic peptide and the corresponding sequence in the native molecule share some immunological epitopes. With computer graphics and energy refinement a molecular model of this peptide has been elaborated.

Original languageEnglish
Pages (from-to)118-122
Number of pages5
JournalFEBS Letters
Volume228
Issue number1
DOIs
Publication statusPublished - Feb 8 1988

Fingerprint

Structural Models
Nicotinic Receptors
Torpedo
Cholinergic Agents
Binding Sites
Peptides
Computer Graphics
Molecular Models
Computer graphics
Epitopes
Amino Acid Sequence
Monoclonal Antibodies
Efficiency
Amino Acids
Molecules
Antibodies

Keywords

  • Computer graphics
  • Molecular modeling
  • Monoclonal antibody
  • Nicotinic acetylcholine receptor
  • α-Bungarotoxin
  • α-Toxin binding site

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Nicotinic acetylcholine receptor : A structural model for α-subunit peptide 188-201, the putative binding site for cholinergic agents. / Gotti, C.; Frigerio, F.; Bolognesi, M.; Longhi, R.; Racchetti, G.; Clementi, F.

In: FEBS Letters, Vol. 228, No. 1, 08.02.1988, p. 118-122.

Research output: Contribution to journalArticle

Gotti, C. ; Frigerio, F. ; Bolognesi, M. ; Longhi, R. ; Racchetti, G. ; Clementi, F. / Nicotinic acetylcholine receptor : A structural model for α-subunit peptide 188-201, the putative binding site for cholinergic agents. In: FEBS Letters. 1988 ; Vol. 228, No. 1. pp. 118-122.
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