Nicotinic acetylcholine receptor: A structural model for α-subunit peptide 188-201, the putative binding site for cholinergic agents

C. Gotti, F. Frigerio, M. Bolognesi, R. Longhi, G. Racchetti, F. Clementi

Research output: Contribution to journalArticlepeer-review

Abstract

A peptide corresponding to amino acid sequence 188-201 of the α-subunit of Torpedo AChR binds α-Bgtx. The S-S bridge between Cys 192 and 193 is essential for the binding as Tyr in position 189. The same sequence 188-201 corresponding to human AChR, which instead of Tyr has a Thr in position 189, binds α-Bgtx with a much lower efficiency. Monoclonal antibodies raised against Torpedo peptide 188-201 recognize Torpedo AChR and antibodies against Torpedo AChR recognize peptide 188-201 indicating that the synthetic peptide and the corresponding sequence in the native molecule share some immunological epitopes. With computer graphics and energy refinement a molecular model of this peptide has been elaborated.

Original languageEnglish
Pages (from-to)118-122
Number of pages5
JournalFEBS Letters
Volume228
Issue number1
DOIs
Publication statusPublished - Feb 8 1988

Keywords

  • Computer graphics
  • Molecular modeling
  • Monoclonal antibody
  • Nicotinic acetylcholine receptor
  • α-Bungarotoxin
  • α-Toxin binding site

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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