Abstract
A peptide corresponding to amino acid sequence 188-201 of the α-subunit of Torpedo AChR binds α-Bgtx. The S-S bridge between Cys 192 and 193 is essential for the binding as Tyr in position 189. The same sequence 188-201 corresponding to human AChR, which instead of Tyr has a Thr in position 189, binds α-Bgtx with a much lower efficiency. Monoclonal antibodies raised against Torpedo peptide 188-201 recognize Torpedo AChR and antibodies against Torpedo AChR recognize peptide 188-201 indicating that the synthetic peptide and the corresponding sequence in the native molecule share some immunological epitopes. With computer graphics and energy refinement a molecular model of this peptide has been elaborated.
Original language | English |
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Pages (from-to) | 118-122 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 228 |
Issue number | 1 |
DOIs | |
Publication status | Published - Feb 8 1988 |
Keywords
- Computer graphics
- Molecular modeling
- Monoclonal antibody
- Nicotinic acetylcholine receptor
- α-Bungarotoxin
- α-Toxin binding site
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology