Abstract
A peptide corresponding to amino acid sequence 188-201 of the α-subunit of Torpedo AChR binds α-Bgtx. The S-S bridge between Cys 192 and 193 is essential for the binding as Tyr in position 189. The same sequence 188-201 corresponding to human AChR, which instead of Tyr has a Thr in position 189, binds α-Bgtx with a much lower efficiency. Monoclonal antibodies raised against Torpedo peptide 188-201 recognize Torpedo AChR and antibodies against Torpedo AChR recognize peptide 188-201 indicating that the synthetic peptide and the corresponding sequence in the native molecule share some immunological epitopes. With computer graphics and energy refinement a molecular model of this peptide has been elaborated.
| Original language | English |
|---|---|
| Pages (from-to) | 118-122 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 228 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 8 1988 |
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Keywords
- Computer graphics
- Molecular modeling
- Monoclonal antibody
- Nicotinic acetylcholine receptor
- α-Bungarotoxin
- α-Toxin binding site
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Nicotinic acetylcholine receptor : A structural model for α-subunit peptide 188-201, the putative binding site for cholinergic agents. / Gotti, C.; Frigerio, F.; Bolognesi, M.; Longhi, R.; Racchetti, G.; Clementi, F.
In: FEBS Letters, Vol. 228, No. 1, 08.02.1988, p. 118-122.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nicotinic acetylcholine receptor
T2 - A structural model for α-subunit peptide 188-201, the putative binding site for cholinergic agents
AU - Gotti, C.
AU - Frigerio, F.
AU - Bolognesi, M.
AU - Longhi, R.
AU - Racchetti, G.
AU - Clementi, F.
PY - 1988/2/8
Y1 - 1988/2/8
N2 - A peptide corresponding to amino acid sequence 188-201 of the α-subunit of Torpedo AChR binds α-Bgtx. The S-S bridge between Cys 192 and 193 is essential for the binding as Tyr in position 189. The same sequence 188-201 corresponding to human AChR, which instead of Tyr has a Thr in position 189, binds α-Bgtx with a much lower efficiency. Monoclonal antibodies raised against Torpedo peptide 188-201 recognize Torpedo AChR and antibodies against Torpedo AChR recognize peptide 188-201 indicating that the synthetic peptide and the corresponding sequence in the native molecule share some immunological epitopes. With computer graphics and energy refinement a molecular model of this peptide has been elaborated.
AB - A peptide corresponding to amino acid sequence 188-201 of the α-subunit of Torpedo AChR binds α-Bgtx. The S-S bridge between Cys 192 and 193 is essential for the binding as Tyr in position 189. The same sequence 188-201 corresponding to human AChR, which instead of Tyr has a Thr in position 189, binds α-Bgtx with a much lower efficiency. Monoclonal antibodies raised against Torpedo peptide 188-201 recognize Torpedo AChR and antibodies against Torpedo AChR recognize peptide 188-201 indicating that the synthetic peptide and the corresponding sequence in the native molecule share some immunological epitopes. With computer graphics and energy refinement a molecular model of this peptide has been elaborated.
KW - Computer graphics
KW - Molecular modeling
KW - Monoclonal antibody
KW - Nicotinic acetylcholine receptor
KW - α-Bungarotoxin
KW - α-Toxin binding site
UR - http://www.scopus.com/inward/record.url?scp=0024281207&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024281207&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(88)80598-2
DO - 10.1016/0014-5793(88)80598-2
M3 - Article
VL - 228
SP - 118
EP - 122
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -