Nidogen-1 is a novel extracellular ligand for the NKp44 activating receptor

Silvia Gaggero, Maurizio Bruschi, Andrea Petretto, Monica Parodi, Genny Del Zotto, Chiara Lavarello, Carola Prato, Laura Santucci, Alessandra Barbuto, Cristina Bottino, Giovanni Candiano, Alessandro Moretta, Massimo Vitale, Lorenzo Moretta, Claudia Cantoni

Research output: Contribution to journalArticle

Abstract

The release of soluble ligands of activating Natural Killer (NK) cell receptors may represent a regulatory mechanism of NK cell function both in physiologic and in pathologic conditions. Here, we identified the extracellular matrix protein Nidogen-1 (NID1) as a ligand of NKp44, an important activating receptor expressed by activated NK cells. When released as soluble molecule, NID1 regulates NK cell function by modulating NKp44-induced IFN-γ production or cytotoxicity. In particular, it also modulates IFN-γ production induced by Platelet-Derived Growth Factor (PDGF)-DD following NKp44 engagement. We also show that NID1 may be present at the cell surface. In this form or when bound to a solid support (bNID1), NID1 fails to induce NK cell cytotoxicity or cytokine release. However, analysis by mass spectrometry revealed that exposure to bNID1 can induce in human NK cells relevant changes in the proteomic profiles suggesting an effect on different biological processes.

Original languageEnglish
Pages (from-to)e1470730
JournalOncoImmunology
Volume7
Issue number9
DOIs
Publication statusPublished - 2018

Fingerprint

Natural Cytotoxicity Triggering Receptor 2
Natural Killer Cells
Ligands
Natural Killer Cell Receptors
Biological Phenomena
Extracellular Matrix Proteins
Platelet-Derived Growth Factor
Proteomics
Mass Spectrometry
nidogen
Cytokines

Cite this

Nidogen-1 is a novel extracellular ligand for the NKp44 activating receptor. / Gaggero, Silvia; Bruschi, Maurizio; Petretto, Andrea; Parodi, Monica; Zotto, Genny Del; Lavarello, Chiara; Prato, Carola; Santucci, Laura; Barbuto, Alessandra; Bottino, Cristina; Candiano, Giovanni; Moretta, Alessandro; Vitale, Massimo; Moretta, Lorenzo; Cantoni, Claudia.

In: OncoImmunology, Vol. 7, No. 9, 2018, p. e1470730.

Research output: Contribution to journalArticle

Gaggero, Silvia ; Bruschi, Maurizio ; Petretto, Andrea ; Parodi, Monica ; Zotto, Genny Del ; Lavarello, Chiara ; Prato, Carola ; Santucci, Laura ; Barbuto, Alessandra ; Bottino, Cristina ; Candiano, Giovanni ; Moretta, Alessandro ; Vitale, Massimo ; Moretta, Lorenzo ; Cantoni, Claudia. / Nidogen-1 is a novel extracellular ligand for the NKp44 activating receptor. In: OncoImmunology. 2018 ; Vol. 7, No. 9. pp. e1470730.
@article{cca05cee022e42b2bef074d0dd116134,
title = "Nidogen-1 is a novel extracellular ligand for the NKp44 activating receptor",
abstract = "The release of soluble ligands of activating Natural Killer (NK) cell receptors may represent a regulatory mechanism of NK cell function both in physiologic and in pathologic conditions. Here, we identified the extracellular matrix protein Nidogen-1 (NID1) as a ligand of NKp44, an important activating receptor expressed by activated NK cells. When released as soluble molecule, NID1 regulates NK cell function by modulating NKp44-induced IFN-γ production or cytotoxicity. In particular, it also modulates IFN-γ production induced by Platelet-Derived Growth Factor (PDGF)-DD following NKp44 engagement. We also show that NID1 may be present at the cell surface. In this form or when bound to a solid support (bNID1), NID1 fails to induce NK cell cytotoxicity or cytokine release. However, analysis by mass spectrometry revealed that exposure to bNID1 can induce in human NK cells relevant changes in the proteomic profiles suggesting an effect on different biological processes.",
author = "Silvia Gaggero and Maurizio Bruschi and Andrea Petretto and Monica Parodi and Zotto, {Genny Del} and Chiara Lavarello and Carola Prato and Laura Santucci and Alessandra Barbuto and Cristina Bottino and Giovanni Candiano and Alessandro Moretta and Massimo Vitale and Lorenzo Moretta and Claudia Cantoni",
year = "2018",
doi = "10.1080/2162402X.2018.1470730",
language = "English",
volume = "7",
pages = "e1470730",
journal = "OncoImmunology",
issn = "2162-4011",
publisher = "Taylor and Francis Inc.",
number = "9",

}

TY - JOUR

T1 - Nidogen-1 is a novel extracellular ligand for the NKp44 activating receptor

AU - Gaggero, Silvia

AU - Bruschi, Maurizio

AU - Petretto, Andrea

AU - Parodi, Monica

AU - Zotto, Genny Del

AU - Lavarello, Chiara

AU - Prato, Carola

AU - Santucci, Laura

AU - Barbuto, Alessandra

AU - Bottino, Cristina

AU - Candiano, Giovanni

AU - Moretta, Alessandro

AU - Vitale, Massimo

AU - Moretta, Lorenzo

AU - Cantoni, Claudia

PY - 2018

Y1 - 2018

N2 - The release of soluble ligands of activating Natural Killer (NK) cell receptors may represent a regulatory mechanism of NK cell function both in physiologic and in pathologic conditions. Here, we identified the extracellular matrix protein Nidogen-1 (NID1) as a ligand of NKp44, an important activating receptor expressed by activated NK cells. When released as soluble molecule, NID1 regulates NK cell function by modulating NKp44-induced IFN-γ production or cytotoxicity. In particular, it also modulates IFN-γ production induced by Platelet-Derived Growth Factor (PDGF)-DD following NKp44 engagement. We also show that NID1 may be present at the cell surface. In this form or when bound to a solid support (bNID1), NID1 fails to induce NK cell cytotoxicity or cytokine release. However, analysis by mass spectrometry revealed that exposure to bNID1 can induce in human NK cells relevant changes in the proteomic profiles suggesting an effect on different biological processes.

AB - The release of soluble ligands of activating Natural Killer (NK) cell receptors may represent a regulatory mechanism of NK cell function both in physiologic and in pathologic conditions. Here, we identified the extracellular matrix protein Nidogen-1 (NID1) as a ligand of NKp44, an important activating receptor expressed by activated NK cells. When released as soluble molecule, NID1 regulates NK cell function by modulating NKp44-induced IFN-γ production or cytotoxicity. In particular, it also modulates IFN-γ production induced by Platelet-Derived Growth Factor (PDGF)-DD following NKp44 engagement. We also show that NID1 may be present at the cell surface. In this form or when bound to a solid support (bNID1), NID1 fails to induce NK cell cytotoxicity or cytokine release. However, analysis by mass spectrometry revealed that exposure to bNID1 can induce in human NK cells relevant changes in the proteomic profiles suggesting an effect on different biological processes.

U2 - 10.1080/2162402X.2018.1470730

DO - 10.1080/2162402X.2018.1470730

M3 - Article

C2 - 30228939

VL - 7

SP - e1470730

JO - OncoImmunology

JF - OncoImmunology

SN - 2162-4011

IS - 9

ER -