NIR-labeled nanoparticles engineered for brain targeting: In vivo optical imaging application and fluorescent microscopy evidences

G. Tosi, L. Bondioli, B. Ruozi, L. Badiali, G. M. Severini, S. Biffi, A. De Vita, B. Bortot, D. Dolcetta, F. Forni, M. A. Vandelli

Research output: Contribution to journalArticle

Abstract

The presence of the blood-brain barrier (BBB) makes extremely difficult to develop efficacious strategies for targeting contrast agents and delivering drugs inside the Central Nervous System (CNS). To overcome this drawback, several kinds of CNS-targeted nanoparticles (NPs) have been developed. In particular, we proposed poly-lactide-co-glycolide (PLGA) NPs engineered with a simil-opioid glycopeptide (g7), which have already proved to be a promising tool for achieving a successful brain targeting after i.v. administration in rats. In order to obtain CNS-targeted NPs to use for in vivo imaging, we synthesized and administrated in mice PLGA NPs with double coverage: near-infrared (NIR) probe (DY-675) and g 7. The optical imaging clearly showed a brain localization of these novel NPs. Thus, a novel kind of NIR-labeled NPs were obtained, providing a new, in vivo detectable nanotechnology tool. Besides, the confocal and fluorescence microscopy evidences allowed to further confirm the ability of g 7 to promote not only the rat, but also the mouse BBB crossing.

Original languageEnglish
Pages (from-to)145-153
Number of pages9
JournalJournal of Neural Transmission
Volume118
Issue number1
DOIs
Publication statusPublished - Jan 2011

Keywords

  • Brain targeting
  • Confocal and fluorescence microscopy
  • Nanoparticles
  • NIR
  • Optical imaging

ASJC Scopus subject areas

  • Biological Psychiatry
  • Neurology
  • Clinical Neurology
  • Psychiatry and Mental health

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