Nitric oxide activates PI3-K and MAPK signalling pathways in human and rat vascular smooth muscle cells: Influence of insulin resistance and oxidative stress

Gabriella Doronzo, Michela Viretto, Isabella Russo, Luigi Mattiello, Leonardo Di Martino, Franco Cavalot, Giovanni Anfossi, Mariella Trovati

Research output: Contribution to journalArticle

Abstract

Objective: Vascular smooth muscle cells (VSMCs) from the animal model of insulin resistance obese Zucker rats (OZR) show impaired ability of nitric oxide (NO) to increase cGMP and of cGMP to activate its specific kinase PKG, these defects being attributable to oxidative stress. We aimed to investigate the intracellular signalling downstream PKG in human and rat VSMC, and to clarify whether it is modified by insulin resistance and oxidative stress. Methods: In aortic VSMC from humans, lean Zucker rats (LZR) and OZR, we measured by Western blots the activation induced by NO and cGMP of signalling molecules of PI3-K and MAPK pathways, with or without PKG inhibition, hydrogen peroxide and antioxidants. We explored the mechanism of the increased oxidative stress in VSMC from OZR by measuring superoxide anion concentrations (luminescence method) with or without inhibition of NADPH oxidase, xanthine oxidase, and mitochondrial electron transport chain complex and by measuring superoxide dismutase (SOD) expression (Western blot) and activity. Results: In VSMC from humans and LZR, the NO/cGMP/PKG pathway activates both PI3-K (Akt, mTOR) and MAPK (ERK-1/2, p38MAPK) signalling. This effect is attenuated in VSMC from OZR, in which the greater oxidative stress is mediated by NADPH oxidase and mitochondrial complex and by a reduced synthesis/activity of SOD. Impairment of the NO/cGMP/PKG signalling is reproduced in VSMC from LZR by hydrogen peroxide and reverted in VSMC from OZR by antioxidants. Conclusions: In VSMC from an animal model of insulin resistance the NO/cGMP/PKG intracellular signalling is impaired due to an increased oxidative stress.

Original languageEnglish
Pages (from-to)44-53
Number of pages10
JournalAtherosclerosis
Volume216
Issue number1
DOIs
Publication statusPublished - May 2011

Fingerprint

Zucker Rats
Vascular Smooth Muscle
Smooth Muscle Myocytes
Insulin Resistance
Nitric Oxide
Oxidative Stress
NADPH Oxidase
Hydrogen Peroxide
Superoxide Dismutase
Animal Models
Antioxidants
Western Blotting
Xanthine Oxidase
Electron Transport
Luminescence
Superoxides
Phosphotransferases

Keywords

  • CGMP
  • Insulin resistance
  • MAPK
  • Nitric oxide
  • Oxidative stress
  • PI3-K
  • PKG
  • Vascular smooth muscle cells
  • Zucker rats

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Nitric oxide activates PI3-K and MAPK signalling pathways in human and rat vascular smooth muscle cells : Influence of insulin resistance and oxidative stress. / Doronzo, Gabriella; Viretto, Michela; Russo, Isabella; Mattiello, Luigi; Di Martino, Leonardo; Cavalot, Franco; Anfossi, Giovanni; Trovati, Mariella.

In: Atherosclerosis, Vol. 216, No. 1, 05.2011, p. 44-53.

Research output: Contribution to journalArticle

Doronzo, Gabriella ; Viretto, Michela ; Russo, Isabella ; Mattiello, Luigi ; Di Martino, Leonardo ; Cavalot, Franco ; Anfossi, Giovanni ; Trovati, Mariella. / Nitric oxide activates PI3-K and MAPK signalling pathways in human and rat vascular smooth muscle cells : Influence of insulin resistance and oxidative stress. In: Atherosclerosis. 2011 ; Vol. 216, No. 1. pp. 44-53.
@article{6604ee9577dc418b8741ed3f3d4cd054,
title = "Nitric oxide activates PI3-K and MAPK signalling pathways in human and rat vascular smooth muscle cells: Influence of insulin resistance and oxidative stress",
abstract = "Objective: Vascular smooth muscle cells (VSMCs) from the animal model of insulin resistance obese Zucker rats (OZR) show impaired ability of nitric oxide (NO) to increase cGMP and of cGMP to activate its specific kinase PKG, these defects being attributable to oxidative stress. We aimed to investigate the intracellular signalling downstream PKG in human and rat VSMC, and to clarify whether it is modified by insulin resistance and oxidative stress. Methods: In aortic VSMC from humans, lean Zucker rats (LZR) and OZR, we measured by Western blots the activation induced by NO and cGMP of signalling molecules of PI3-K and MAPK pathways, with or without PKG inhibition, hydrogen peroxide and antioxidants. We explored the mechanism of the increased oxidative stress in VSMC from OZR by measuring superoxide anion concentrations (luminescence method) with or without inhibition of NADPH oxidase, xanthine oxidase, and mitochondrial electron transport chain complex and by measuring superoxide dismutase (SOD) expression (Western blot) and activity. Results: In VSMC from humans and LZR, the NO/cGMP/PKG pathway activates both PI3-K (Akt, mTOR) and MAPK (ERK-1/2, p38MAPK) signalling. This effect is attenuated in VSMC from OZR, in which the greater oxidative stress is mediated by NADPH oxidase and mitochondrial complex and by a reduced synthesis/activity of SOD. Impairment of the NO/cGMP/PKG signalling is reproduced in VSMC from LZR by hydrogen peroxide and reverted in VSMC from OZR by antioxidants. Conclusions: In VSMC from an animal model of insulin resistance the NO/cGMP/PKG intracellular signalling is impaired due to an increased oxidative stress.",
keywords = "CGMP, Insulin resistance, MAPK, Nitric oxide, Oxidative stress, PI3-K, PKG, Vascular smooth muscle cells, Zucker rats",
author = "Gabriella Doronzo and Michela Viretto and Isabella Russo and Luigi Mattiello and {Di Martino}, Leonardo and Franco Cavalot and Giovanni Anfossi and Mariella Trovati",
year = "2011",
month = "5",
doi = "10.1016/j.atherosclerosis.2011.01.019",
language = "English",
volume = "216",
pages = "44--53",
journal = "Atherosclerosis",
issn = "0021-9150",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Nitric oxide activates PI3-K and MAPK signalling pathways in human and rat vascular smooth muscle cells

T2 - Influence of insulin resistance and oxidative stress

AU - Doronzo, Gabriella

AU - Viretto, Michela

AU - Russo, Isabella

AU - Mattiello, Luigi

AU - Di Martino, Leonardo

AU - Cavalot, Franco

AU - Anfossi, Giovanni

AU - Trovati, Mariella

PY - 2011/5

Y1 - 2011/5

N2 - Objective: Vascular smooth muscle cells (VSMCs) from the animal model of insulin resistance obese Zucker rats (OZR) show impaired ability of nitric oxide (NO) to increase cGMP and of cGMP to activate its specific kinase PKG, these defects being attributable to oxidative stress. We aimed to investigate the intracellular signalling downstream PKG in human and rat VSMC, and to clarify whether it is modified by insulin resistance and oxidative stress. Methods: In aortic VSMC from humans, lean Zucker rats (LZR) and OZR, we measured by Western blots the activation induced by NO and cGMP of signalling molecules of PI3-K and MAPK pathways, with or without PKG inhibition, hydrogen peroxide and antioxidants. We explored the mechanism of the increased oxidative stress in VSMC from OZR by measuring superoxide anion concentrations (luminescence method) with or without inhibition of NADPH oxidase, xanthine oxidase, and mitochondrial electron transport chain complex and by measuring superoxide dismutase (SOD) expression (Western blot) and activity. Results: In VSMC from humans and LZR, the NO/cGMP/PKG pathway activates both PI3-K (Akt, mTOR) and MAPK (ERK-1/2, p38MAPK) signalling. This effect is attenuated in VSMC from OZR, in which the greater oxidative stress is mediated by NADPH oxidase and mitochondrial complex and by a reduced synthesis/activity of SOD. Impairment of the NO/cGMP/PKG signalling is reproduced in VSMC from LZR by hydrogen peroxide and reverted in VSMC from OZR by antioxidants. Conclusions: In VSMC from an animal model of insulin resistance the NO/cGMP/PKG intracellular signalling is impaired due to an increased oxidative stress.

AB - Objective: Vascular smooth muscle cells (VSMCs) from the animal model of insulin resistance obese Zucker rats (OZR) show impaired ability of nitric oxide (NO) to increase cGMP and of cGMP to activate its specific kinase PKG, these defects being attributable to oxidative stress. We aimed to investigate the intracellular signalling downstream PKG in human and rat VSMC, and to clarify whether it is modified by insulin resistance and oxidative stress. Methods: In aortic VSMC from humans, lean Zucker rats (LZR) and OZR, we measured by Western blots the activation induced by NO and cGMP of signalling molecules of PI3-K and MAPK pathways, with or without PKG inhibition, hydrogen peroxide and antioxidants. We explored the mechanism of the increased oxidative stress in VSMC from OZR by measuring superoxide anion concentrations (luminescence method) with or without inhibition of NADPH oxidase, xanthine oxidase, and mitochondrial electron transport chain complex and by measuring superoxide dismutase (SOD) expression (Western blot) and activity. Results: In VSMC from humans and LZR, the NO/cGMP/PKG pathway activates both PI3-K (Akt, mTOR) and MAPK (ERK-1/2, p38MAPK) signalling. This effect is attenuated in VSMC from OZR, in which the greater oxidative stress is mediated by NADPH oxidase and mitochondrial complex and by a reduced synthesis/activity of SOD. Impairment of the NO/cGMP/PKG signalling is reproduced in VSMC from LZR by hydrogen peroxide and reverted in VSMC from OZR by antioxidants. Conclusions: In VSMC from an animal model of insulin resistance the NO/cGMP/PKG intracellular signalling is impaired due to an increased oxidative stress.

KW - CGMP

KW - Insulin resistance

KW - MAPK

KW - Nitric oxide

KW - Oxidative stress

KW - PI3-K

KW - PKG

KW - Vascular smooth muscle cells

KW - Zucker rats

UR - http://www.scopus.com/inward/record.url?scp=79955528930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955528930&partnerID=8YFLogxK

U2 - 10.1016/j.atherosclerosis.2011.01.019

DO - 10.1016/j.atherosclerosis.2011.01.019

M3 - Article

C2 - 21316056

AN - SCOPUS:79955528930

VL - 216

SP - 44

EP - 53

JO - Atherosclerosis

JF - Atherosclerosis

SN - 0021-9150

IS - 1

ER -