Nitric oxide deficiency determines global chromatin changes in Duchenne muscular dystrophy

Claudia Colussi, Aymone Gurtner, Jessica Rosati, Barbara Illi, Gianluca Ragone, Giulia Piaggio, Maurizio Moggio, Costanza Lamperti, Grazia D'Angelo, Emilio Clementi, Giulia Minetti, Chiara Mozzetta, Annalisa Antonini, Maurizio C. Capogrossi, Pier Lorenzo Puri, Carlo Gaetano

Research output: Contribution to journalArticle

Abstract

The present study provides evidence that abnormal patterns of global histone modification are present in the skeletal muscle nuclei of mdx mice and Duchenne muscular dystrophy (DMD) patients. A combination of specific histone H3 modifications, including Ser-10 phosphorylation, acetylation of Lys 9 and 14, and Lys 79 methylation, were found enriched in muscle biopsies from human patients affected by DMD and in lateterm fetuses, early postnatal pups, or adult mdx mice. In this context, chromatin immunoprecipitation experiments showed an enrichment of these modifications at the loci of genes involved in proliferation or inflammation, suggesting a regulatory effect on gene expression. Remarkably, the reexpression of dystrophin induced by gentamicin treatment or the administration of nitric oxide (NO) donors reversed the abnormal pattern of H3 histone modifications. These findings suggest an unanticipated link between the dystrophin-activated NO signaling and the remodeling of chromatin. In this context, the regulation of class IIa histone deacetylases (HDACs) 4 and 5 was found altered as a consequence of the reduced NO-dependent protein phosphatase 2A activity, indicating that bothNOand class IIa HDACs are important for satellite cell differentiation and gene expression in mdx mice. In conclusion, this work provides the first evidence of a role for NO as an epigenetic regulator in DMD.

Original languageEnglish
Pages (from-to)2131-2141
Number of pages11
JournalFASEB Journal
Volume23
Issue number7
DOIs
Publication statusPublished - Jul 2009

Fingerprint

Histone Code
Inbred mdx Mouse
Duchenne Muscular Dystrophy
Histones
Chromatin
Dystrophin
Nitric Oxide
Histone Deacetylases
Gene expression
Muscle
Gene Expression
Protein Phosphatase 2
Acetylation
Phosphorylation
Methylation
Chromatin Assembly and Disassembly
Nitric Oxide Donors
Biopsy
Chromatin Immunoprecipitation
Gentamicins

Keywords

  • Differentiation
  • Histone
  • Histone deacetylase
  • Myoblast
  • Protein phosphatase

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology
  • Medicine(all)

Cite this

Nitric oxide deficiency determines global chromatin changes in Duchenne muscular dystrophy. / Colussi, Claudia; Gurtner, Aymone; Rosati, Jessica; Illi, Barbara; Ragone, Gianluca; Piaggio, Giulia; Moggio, Maurizio; Lamperti, Costanza; D'Angelo, Grazia; Clementi, Emilio; Minetti, Giulia; Mozzetta, Chiara; Antonini, Annalisa; Capogrossi, Maurizio C.; Puri, Pier Lorenzo; Gaetano, Carlo.

In: FASEB Journal, Vol. 23, No. 7, 07.2009, p. 2131-2141.

Research output: Contribution to journalArticle

Colussi, C, Gurtner, A, Rosati, J, Illi, B, Ragone, G, Piaggio, G, Moggio, M, Lamperti, C, D'Angelo, G, Clementi, E, Minetti, G, Mozzetta, C, Antonini, A, Capogrossi, MC, Puri, PL & Gaetano, C 2009, 'Nitric oxide deficiency determines global chromatin changes in Duchenne muscular dystrophy', FASEB Journal, vol. 23, no. 7, pp. 2131-2141. https://doi.org/10.1096/fj.08-115618
Colussi, Claudia ; Gurtner, Aymone ; Rosati, Jessica ; Illi, Barbara ; Ragone, Gianluca ; Piaggio, Giulia ; Moggio, Maurizio ; Lamperti, Costanza ; D'Angelo, Grazia ; Clementi, Emilio ; Minetti, Giulia ; Mozzetta, Chiara ; Antonini, Annalisa ; Capogrossi, Maurizio C. ; Puri, Pier Lorenzo ; Gaetano, Carlo. / Nitric oxide deficiency determines global chromatin changes in Duchenne muscular dystrophy. In: FASEB Journal. 2009 ; Vol. 23, No. 7. pp. 2131-2141.
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