Nitric oxide independent activation of guanylate cyclase by YC-1 causes erectile responses in the rat

H. Mizusawa, P. Hedlund, J. D. Brioni, J. P. Sullivan, K. E. Andersson

Research output: Contribution to journalArticle

Abstract

Purpose: Activation of soluble guanylate cyclase with a subsequent increase in intracellular levels of cyclic guanosine monophosphate is necessary for normal erection. In vascular tissue 3(5′-hydroxymethyl-2′-furyl-l-benzyl indazole (YC-1) (Abbott Laboratories, North Chicago, Illinois) has been shown to stimulate soluble guanylate cyclase independent of nitric oxide. We studied whether YC-1 modulates erectile responses in the rat. Materials and Methods: The effects of YC-1 given intracavernously or intraperitoneally on intracavernous pressure were investigated in rats. Functional effects of YC-1 on neuronal and endothelial nitric oxide relaxations were studied in 3 × 10-6 M. 1-noradrenaline contracted preparations of rat isolated corpus cavernosum. Results: Intracavernous YC-1 (10 μmol. kg.-1) produced erectile responses with a mean intracavernous pressure plus or minus standard error of mean of 81 ± 17 cm. water (p -1 given intraperitoneally also increased the amplitude and duration of erectile responses to cavernous nerve stimulation. Mean peak intracavernous pressure increased from 63 ± 6 to 102 ± 16 cm. water (p -1 apomorphine s.c. increased in number after 10 μmol. kg.-1 YC-1 intraperitoneally (p -5 M. (p

Original languageEnglish
Pages (from-to)2276-2281
Number of pages6
JournalJournal of Urology
Volume167
Issue number5
Publication statusPublished - 2002

Keywords

  • Guanylate cyclase
  • Penile erection
  • Penis
  • Rats, Sprague-Dawley

ASJC Scopus subject areas

  • Urology

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    Mizusawa, H., Hedlund, P., Brioni, J. D., Sullivan, J. P., & Andersson, K. E. (2002). Nitric oxide independent activation of guanylate cyclase by YC-1 causes erectile responses in the rat. Journal of Urology, 167(5), 2276-2281.