Nitric oxide independent activation of guanylate cyclase by YC-1 causes erectile responses in the rat

H. Mizusawa, P. Hedlund, J. D. Brioni, J. P. Sullivan, K. E. Andersson

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Purpose: Activation of soluble guanylate cyclase with a subsequent increase in intracellular levels of cyclic guanosine monophosphate is necessary for normal erection. In vascular tissue 3(5′-hydroxymethyl-2′-furyl-l-benzyl indazole (YC-1) (Abbott Laboratories, North Chicago, Illinois) has been shown to stimulate soluble guanylate cyclase independent of nitric oxide. We studied whether YC-1 modulates erectile responses in the rat. Materials and Methods: The effects of YC-1 given intracavernously or intraperitoneally on intracavernous pressure were investigated in rats. Functional effects of YC-1 on neuronal and endothelial nitric oxide relaxations were studied in 3 × 10-6 M. 1-noradrenaline contracted preparations of rat isolated corpus cavernosum. Results: Intracavernous YC-1 (10 μmol. kg.-1) produced erectile responses with a mean intracavernous pressure plus or minus standard error of mean of 81 ± 17 cm. water (p -1 given intraperitoneally also increased the amplitude and duration of erectile responses to cavernous nerve stimulation. Mean peak intracavernous pressure increased from 63 ± 6 to 102 ± 16 cm. water (p -1 apomorphine s.c. increased in number after 10 μmol. kg.-1 YC-1 intraperitoneally (p -5 M. (p

Original languageEnglish
Pages (from-to)2276-2281
Number of pages6
JournalJournal of Urology
Volume167
Issue number5
Publication statusPublished - 2002

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Nitric Oxide
Pressure
Indazoles
Water
Apomorphine
Cyclic GMP
Blood Vessels
Norepinephrine
guanylate cyclase 1
Soluble Guanylyl Cyclase

Keywords

  • Guanylate cyclase
  • Penile erection
  • Penis
  • Rats, Sprague-Dawley

ASJC Scopus subject areas

  • Urology

Cite this

Mizusawa, H., Hedlund, P., Brioni, J. D., Sullivan, J. P., & Andersson, K. E. (2002). Nitric oxide independent activation of guanylate cyclase by YC-1 causes erectile responses in the rat. Journal of Urology, 167(5), 2276-2281.

Nitric oxide independent activation of guanylate cyclase by YC-1 causes erectile responses in the rat. / Mizusawa, H.; Hedlund, P.; Brioni, J. D.; Sullivan, J. P.; Andersson, K. E.

In: Journal of Urology, Vol. 167, No. 5, 2002, p. 2276-2281.

Research output: Contribution to journalArticle

Mizusawa, H, Hedlund, P, Brioni, JD, Sullivan, JP & Andersson, KE 2002, 'Nitric oxide independent activation of guanylate cyclase by YC-1 causes erectile responses in the rat', Journal of Urology, vol. 167, no. 5, pp. 2276-2281.
Mizusawa H, Hedlund P, Brioni JD, Sullivan JP, Andersson KE. Nitric oxide independent activation of guanylate cyclase by YC-1 causes erectile responses in the rat. Journal of Urology. 2002;167(5):2276-2281.
Mizusawa, H. ; Hedlund, P. ; Brioni, J. D. ; Sullivan, J. P. ; Andersson, K. E. / Nitric oxide independent activation of guanylate cyclase by YC-1 causes erectile responses in the rat. In: Journal of Urology. 2002 ; Vol. 167, No. 5. pp. 2276-2281.
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