TY - JOUR
T1 - Nitric oxide inhibits HIV-1 replication in human astrocytoma cells
AU - Persichini, Tiziana
AU - Colasanti, Marco
AU - Fraziano, Maurizio
AU - Colizzi, Vittorio
AU - Ascenzi, Paolo
AU - Lauro, Giuliana M.
PY - 1999/1/8
Y1 - 1999/1/8
N2 - Astroglial cells represent a target for HIV infection in the central nervous system. In astrocytes, HIV infection is poorly productive, being characterized by a persistent state of viral latency. However, activation of the nuclear factor NF-κB and its binding to HIV long terminal repeat (LTR) can induce HIV replication. Moreover, nitric oxide (NO) can affect NF-κB activation in glial cells. Therefore, we hypothesize that NO may reduce HIV replication in human astroglial cells by inhibiting HIV-1 LTR transcriptional activity. In this respect, we show that NO donors reduce viral replication in HIV-1-infected human astrocytoma T67 cells, taken as an astroglial model. Furthermore, using transfected T67 cells, we demonstrate that NO donors inhibit HIV-1 LTR transcriptional activity. These results suggest that the use of NO-releasing drugs may represent a potential, novel approach in inhibiting HIV replication in the central nervous system.
AB - Astroglial cells represent a target for HIV infection in the central nervous system. In astrocytes, HIV infection is poorly productive, being characterized by a persistent state of viral latency. However, activation of the nuclear factor NF-κB and its binding to HIV long terminal repeat (LTR) can induce HIV replication. Moreover, nitric oxide (NO) can affect NF-κB activation in glial cells. Therefore, we hypothesize that NO may reduce HIV replication in human astroglial cells by inhibiting HIV-1 LTR transcriptional activity. In this respect, we show that NO donors reduce viral replication in HIV-1-infected human astrocytoma T67 cells, taken as an astroglial model. Furthermore, using transfected T67 cells, we demonstrate that NO donors inhibit HIV-1 LTR transcriptional activity. These results suggest that the use of NO-releasing drugs may represent a potential, novel approach in inhibiting HIV replication in the central nervous system.
KW - AIDS dementia complex
KW - Glial cells
KW - HIV-1 replication
KW - Nitric oxide
UR - http://www.scopus.com/inward/record.url?scp=0033534755&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033534755&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1998.9880
DO - 10.1006/bbrc.1998.9880
M3 - Article
C2 - 9920758
AN - SCOPUS:0033534755
VL - 254
SP - 200
EP - 202
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -