Nitric oxide inhibits HIV-1 replication in human astrocytoma cells

Tiziana Persichini, Marco Colasanti, Maurizio Fraziano, Vittorio Colizzi, Paolo Ascenzi, Giuliana M. Lauro

Research output: Contribution to journalArticlepeer-review

Abstract

Astroglial cells represent a target for HIV infection in the central nervous system. In astrocytes, HIV infection is poorly productive, being characterized by a persistent state of viral latency. However, activation of the nuclear factor NF-κB and its binding to HIV long terminal repeat (LTR) can induce HIV replication. Moreover, nitric oxide (NO) can affect NF-κB activation in glial cells. Therefore, we hypothesize that NO may reduce HIV replication in human astroglial cells by inhibiting HIV-1 LTR transcriptional activity. In this respect, we show that NO donors reduce viral replication in HIV-1-infected human astrocytoma T67 cells, taken as an astroglial model. Furthermore, using transfected T67 cells, we demonstrate that NO donors inhibit HIV-1 LTR transcriptional activity. These results suggest that the use of NO-releasing drugs may represent a potential, novel approach in inhibiting HIV replication in the central nervous system.

Original languageEnglish
Pages (from-to)200-202
Number of pages3
JournalBiochemical and Biophysical Research Communications
Volume254
Issue number1
DOIs
Publication statusPublished - Jan 8 1999

Keywords

  • AIDS dementia complex
  • Glial cells
  • HIV-1 replication
  • Nitric oxide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Nitric oxide inhibits HIV-1 replication in human astrocytoma cells'. Together they form a unique fingerprint.

Cite this