Nitric oxide inhibits selectively the 17β-estradiol-induced gene expression without affecting nongenomic events in HeLa cells

Maria Marino, Ramona Ficca, Paolo Ascenzi, Anna Trentalance

Research output: Contribution to journalArticlepeer-review

Abstract

17β-estradiol (E2) induces genomic (i.e., pC3-luciferase promoter-reporter construct expression) and nongenomic (i.e., DNA synthesis and IP3 production) effects in HeLa cells only after transient transfection with the human estrogen receptor α (ERα) reporter plasmid. Here the effect of nitric oxide (NO) on both E2-induced effects in transiently transfected HeLa cells is reported. Remarkably, the E2-dependent gene transcription is inhibited dose-dependently by NO. By contrast, DNA synthesis and IP3 production, representing nongenomic E2-dependent effects, are unaffected by NO. The selective NO action on E2-induced functions may be related to NO-mediated chemical modification(s) of the Cys residues present in the DNA recognition domain of ERα impairing DNA binding.

Original languageEnglish
Pages (from-to)529-533
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume286
Issue number3
DOIs
Publication statusPublished - 2001

Keywords

  • 17-β-estradiol
  • DNA synthesis
  • Gene transcription inhibition
  • HeLa cells
  • Human estrogen receptor α
  • IP production
  • Nitric oxide
  • Transcriptional activity

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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