Nitric oxide inhibits the HIV-1 reverse transcriptase activity

Tiziana Persichini, Marco Colasanti, Maurizio Fraziano, Vittorio Colizzi, Claudio Medana, Fabio Polticelli, Giorgio Venturini, Paolo Ascenzi

Research output: Contribution to journalArticlepeer-review

Abstract

Nitric oxide (NO) is a polypotent regulatory molecule involved in a variety of activities, such as the modulation of the catalytic activity of cysteine-containing enzymes. The present study reports the modulation of the HIV-1 reverse transcriptase activity by NO, released by the NO-donors 3,3-bis(aminoethyl)-1-hydroxy-2-oxo-1-triazene (NOC-18), (±)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (NOR-3), 3-morpholinosydnonimine (SIN-1), 4-(phenylsulfonyl)-3-((2-(dimethylamino)ethyl)thio)furoxan oxalate (SNO-102), and sodium nitroprusside (SNP). NO inhibits dose-dependently the HIV-1 reverse transcriptase activity, likely due to oxidation of Cys residue(s). Present results, representing a new insight into the modulation mechanism of the HIV-1 reverse transcriptase activity, may be relevant to develop new strategies for inhibition of HIV-1 replication.

Original languageEnglish
Pages (from-to)624-627
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume258
Issue number3
DOIs
Publication statusPublished - May 19 1999

Keywords

  • Enzyme inhibition
  • HIV-1 reverse transcriptase
  • Nitric oxide
  • NO-donor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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