Nitric oxide mediates low magnesium inhibition of osteoblast-like cell proliferation

Marzia Leidi, Federica Dellera, Massimo Mariotti, Giuseppe Banfi, Calogero Crapanzano, Walter Albisetti, Jeanette A M Maier

Research output: Contribution to journalArticlepeer-review

Abstract

An adequate intake of magnesium (Mg) is important for bone cell activity and contributes to the prevention of osteoporosis. Because (a) Mg is mitogenic for osteoblasts and (b) reduction of osteoblast proliferation is detected in osteoporosis, we investigated the influence of different concentrations of extracellular Mg on osteoblast-like SaOS-2 cell behavior. We found that low Mg inhibited SaOS-2 cell proliferation by increasing the release of nitric oxide through the up-regulation of inducible nitric oxide synthase (iNOS). Indeed, both pharmacological inhibition with the iNOS inhibitor l-N6-(iminoethyl)-lysine-HCl and genetic silencing of iNOS by small interfering RNA restored the normal proliferation rate of the cells. Because a moderate induction of nitric oxide is sufficient to potentiate bone resorption and a relative deficiency in osteoblast proliferation can result in their inadequate activity, we conclude that maintaining Mg homeostasis is relevant to ensure osteoblast function and, therefore, to prevent osteoporosis.

Original languageEnglish
Pages (from-to)1224-1229
Number of pages6
JournalJournal of Nutritional Biochemistry
Volume23
Issue number10
DOIs
Publication statusPublished - Oct 2012

Keywords

  • INOS
  • Magnesium
  • Nitric oxide
  • Osteoblast
  • Osteoporosis

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

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