Nitric oxide production in living neurons is modulated by sphingosine: A fluorescence microscopy study

Paola Viani, Paola Giussani, Anita Ferraretto, Alessandra Signorile, Laura Riboni, Guido Tettamanti

Research output: Contribution to journalArticlepeer-review

Abstract

An investigation was carried out into the possible effect of sphingosine (Sph) on nitric oxide (NO) production in living neurons. Differentiated granule cells were used in a dynamic videoimaging analysis of single cells labeled, simultaneously, with FURA-2 and the NO indicator 4,5-diaminofluorescein. The results demonstrate that Sph exerts a potent inhibitory effect on the Ca2+-dependent production of NO, without modifying the [Ca2+]i. The effect appears to be specific as neither ceramide nor Sph-1-phosphate had any effect on the NO and [Ca2+]i levels. The data demonstrate that Ca2+-dependent NO production is a specific Sph target in living granule cells, suggesting that this bioactive sphingoid plays a relevant role in neuronal NO signaling.

Original languageEnglish
Pages (from-to)185-190
Number of pages6
JournalFEBS Letters
Volume506
Issue number3
DOIs
Publication statusPublished - Oct 12 2001

Keywords

  • 4,5-Diaminofluorescein
  • Cerebellar granule cell
  • Dynamic videoimaging
  • Fluorescence indicator
  • Nitric oxide
  • Sphingosine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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