TY - JOUR
T1 - Nitric oxide production in living neurons is modulated by sphingosine
T2 - A fluorescence microscopy study
AU - Viani, Paola
AU - Giussani, Paola
AU - Ferraretto, Anita
AU - Signorile, Alessandra
AU - Riboni, Laura
AU - Tettamanti, Guido
PY - 2001/10/12
Y1 - 2001/10/12
N2 - An investigation was carried out into the possible effect of sphingosine (Sph) on nitric oxide (NO) production in living neurons. Differentiated granule cells were used in a dynamic videoimaging analysis of single cells labeled, simultaneously, with FURA-2 and the NO indicator 4,5-diaminofluorescein. The results demonstrate that Sph exerts a potent inhibitory effect on the Ca2+-dependent production of NO, without modifying the [Ca2+]i. The effect appears to be specific as neither ceramide nor Sph-1-phosphate had any effect on the NO and [Ca2+]i levels. The data demonstrate that Ca2+-dependent NO production is a specific Sph target in living granule cells, suggesting that this bioactive sphingoid plays a relevant role in neuronal NO signaling.
AB - An investigation was carried out into the possible effect of sphingosine (Sph) on nitric oxide (NO) production in living neurons. Differentiated granule cells were used in a dynamic videoimaging analysis of single cells labeled, simultaneously, with FURA-2 and the NO indicator 4,5-diaminofluorescein. The results demonstrate that Sph exerts a potent inhibitory effect on the Ca2+-dependent production of NO, without modifying the [Ca2+]i. The effect appears to be specific as neither ceramide nor Sph-1-phosphate had any effect on the NO and [Ca2+]i levels. The data demonstrate that Ca2+-dependent NO production is a specific Sph target in living granule cells, suggesting that this bioactive sphingoid plays a relevant role in neuronal NO signaling.
KW - 4,5-Diaminofluorescein
KW - Cerebellar granule cell
KW - Dynamic videoimaging
KW - Fluorescence indicator
KW - Nitric oxide
KW - Sphingosine
UR - http://www.scopus.com/inward/record.url?scp=0035850894&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035850894&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(01)02880-0
DO - 10.1016/S0014-5793(01)02880-0
M3 - Article
C2 - 11602242
AN - SCOPUS:0035850894
VL - 506
SP - 185
EP - 190
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -