Abstract
A specific and simple method for the direct simultaneous detection of extracellular nitrite (NO 2 -) and nitrate (NO 3 -) has been developed, using high-performance liquid chromatography separation with UV and electrochemical detection in series. These stable endproducts of nitric oxide (NO·) were determined in dialysis perfusate obtained through in vivo brain microdialysis during and after experimental photoinduced cerebral ischemia in rats. The chromatographic conditions were optimized with a reversed-phase column (250X46 mm) using 10 mM n=octylamine pH 6.0 as a mobile phase. Absorbance was measured at 220 nm for NO 3 - detection; electrochemical detection was performed at +0.7 V for NO 2 - evaluation. This assay system holds the advantages of in vivo consecutive measurements, high precision, good reproducibility, technical simplicity, fast response (about 7 min), and wide availability.
| Original language | English |
|---|---|
| Pages (from-to) | 103-108 |
| Number of pages | 6 |
| Journal | Journal of Chromatography A |
| Volume | 798 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Mar 6 1998 |
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Keywords
- Inorganic anions
- Neurotransmitters
- Nitrate
- Nitric oxide
- Nitrite
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Nitrite/nitrate balance during photoinduced cerebral ischemia in the rat determined by high-performance liquid chromatography with uv and electrochemical detection. / Rizzo, V.; Montalbetti, L.; Rozza, A. L.; Bolzani, W.; Porta, C.; Balduzzi, G.; Scoglio, E.; Moratti, R.
In: Journal of Chromatography A, Vol. 798, No. 1-2, 06.03.1998, p. 103-108.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nitrite/nitrate balance during photoinduced cerebral ischemia in the rat determined by high-performance liquid chromatography with uv and electrochemical detection
AU - Rizzo, V.
AU - Montalbetti, L.
AU - Rozza, A. L.
AU - Bolzani, W.
AU - Porta, C.
AU - Balduzzi, G.
AU - Scoglio, E.
AU - Moratti, R.
PY - 1998/3/6
Y1 - 1998/3/6
N2 - A specific and simple method for the direct simultaneous detection of extracellular nitrite (NO 2 -) and nitrate (NO 3 -) has been developed, using high-performance liquid chromatography separation with UV and electrochemical detection in series. These stable endproducts of nitric oxide (NO·) were determined in dialysis perfusate obtained through in vivo brain microdialysis during and after experimental photoinduced cerebral ischemia in rats. The chromatographic conditions were optimized with a reversed-phase column (250X46 mm) using 10 mM n=octylamine pH 6.0 as a mobile phase. Absorbance was measured at 220 nm for NO 3 - detection; electrochemical detection was performed at +0.7 V for NO 2 - evaluation. This assay system holds the advantages of in vivo consecutive measurements, high precision, good reproducibility, technical simplicity, fast response (about 7 min), and wide availability.
AB - A specific and simple method for the direct simultaneous detection of extracellular nitrite (NO 2 -) and nitrate (NO 3 -) has been developed, using high-performance liquid chromatography separation with UV and electrochemical detection in series. These stable endproducts of nitric oxide (NO·) were determined in dialysis perfusate obtained through in vivo brain microdialysis during and after experimental photoinduced cerebral ischemia in rats. The chromatographic conditions were optimized with a reversed-phase column (250X46 mm) using 10 mM n=octylamine pH 6.0 as a mobile phase. Absorbance was measured at 220 nm for NO 3 - detection; electrochemical detection was performed at +0.7 V for NO 2 - evaluation. This assay system holds the advantages of in vivo consecutive measurements, high precision, good reproducibility, technical simplicity, fast response (about 7 min), and wide availability.
KW - Inorganic anions
KW - Neurotransmitters
KW - Nitrate
KW - Nitric oxide
KW - Nitrite
UR - http://www.scopus.com/inward/record.url?scp=18244424190&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=18244424190&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(97)01186-2
DO - 10.1016/S0021-9673(97)01186-2
M3 - Article
C2 - 9542131
AN - SCOPUS:18244424190
VL - 798
SP - 103
EP - 108
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1-2
ER -