Nitrite/nitrate balance during photoinduced cerebral ischemia in the rat determined by high-performance liquid chromatography with uv and electrochemical detection

V. Rizzo, L. Montalbetti, A. L. Rozza, W. Bolzani, C. Porta, G. Balduzzi, E. Scoglio, R. Moratti

Research output: Contribution to journalArticlepeer-review

Abstract

A specific and simple method for the direct simultaneous detection of extracellular nitrite (NO 2 -) and nitrate (NO 3 -) has been developed, using high-performance liquid chromatography separation with UV and electrochemical detection in series. These stable endproducts of nitric oxide (NO·) were determined in dialysis perfusate obtained through in vivo brain microdialysis during and after experimental photoinduced cerebral ischemia in rats. The chromatographic conditions were optimized with a reversed-phase column (250X46 mm) using 10 mM n=octylamine pH 6.0 as a mobile phase. Absorbance was measured at 220 nm for NO 3 - detection; electrochemical detection was performed at +0.7 V for NO 2 - evaluation. This assay system holds the advantages of in vivo consecutive measurements, high precision, good reproducibility, technical simplicity, fast response (about 7 min), and wide availability.

Original languageEnglish
Pages (from-to)103-108
Number of pages6
JournalJournal of Chromatography A
Volume798
Issue number1-2
DOIs
Publication statusPublished - Mar 6 1998

Keywords

  • Inorganic anions
  • Neurotransmitters
  • Nitrate
  • Nitric oxide
  • Nitrite

ASJC Scopus subject areas

  • Analytical Chemistry

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