NKp44 expression, phylogenesis and function in non-human primate NK cells

Andrea De Maria, Elisabetta Ugolotti, Erik Rutjens, Stefania Mazza, Luana Radic, Alessandro Faravelli, Gerrit Koopman, Eddi Di Marco, Paola Costa, Barbara Ensoli, Aurelio Cafaro, Maria Cristina Mingari, Lorenzo Moretta, Jonathan Heeney, Roberto Biassoni

Research output: Contribution to journalArticle

Abstract

Molecular and functional characterization of the natural cytotoxicity receptor (NCR) NKp44 in species other than Homo sapiens has been elusive, so far. Here, we provide complete phenotypic, molecular and functional characterization for NKp44 triggering receptor on Pan troglodytes NK cells, the closest human relative, and the analysis of NKp44-genomic locus and transcription in Macaca fascicularis. Similar to H. sapiens, NKp44 expression is detectable on chimpanzee NK cells only upon activation. However, basal NKp44 transcription is 5-fold higher in chimpanzees with lower differential increases upon cell activation compared with humans. Upon activation, an overall 12-fold lower NKp44 gene expression is observed in P. troglodytes compared with H. sapiens NK cells with only a slight reduction in NKp44 surface expression. Functional analysis of 'in vitro' activated purified NK cells confirms the NKp44 triggering potential compared with other major NCRs. These findings suggest the presence of a post-transcriptional regulation that evolved differently in H. sapiens. Analysis of cynomolgus NKp44-genomic sequence and transcription pattern showed very low levels of transcription with occurrence of out-of-frame transcripts and no surface expression. The present comparative analysis suggests that NKp44-genomic organization appears during macaque speciation, with considerable evolution of its transcriptional and post-transcriptional tuning. Thus, NKp44 may represent an NCR being only recently emerged during speciation, acquiring functional relevance only in non-human primates closest to H. sapiens.

Original languageEnglish
Pages (from-to)245-255
Number of pages11
JournalInternational Immunology
Volume21
Issue number3
DOIs
Publication statusPublished - 2009

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Phylogeny
Natural Killer Cells
Primates
Pan troglodytes
Natural Cytotoxicity Triggering Receptor 2
Macaca fascicularis
Macaca
Gene Expression

Keywords

  • Cytotoxicity
  • Evolution
  • Molecular biology
  • Natural cytotoxicity receptors

ASJC Scopus subject areas

  • Immunology

Cite this

NKp44 expression, phylogenesis and function in non-human primate NK cells. / De Maria, Andrea; Ugolotti, Elisabetta; Rutjens, Erik; Mazza, Stefania; Radic, Luana; Faravelli, Alessandro; Koopman, Gerrit; Di Marco, Eddi; Costa, Paola; Ensoli, Barbara; Cafaro, Aurelio; Mingari, Maria Cristina; Moretta, Lorenzo; Heeney, Jonathan; Biassoni, Roberto.

In: International Immunology, Vol. 21, No. 3, 2009, p. 245-255.

Research output: Contribution to journalArticle

De Maria, A, Ugolotti, E, Rutjens, E, Mazza, S, Radic, L, Faravelli, A, Koopman, G, Di Marco, E, Costa, P, Ensoli, B, Cafaro, A, Mingari, MC, Moretta, L, Heeney, J & Biassoni, R 2009, 'NKp44 expression, phylogenesis and function in non-human primate NK cells', International Immunology, vol. 21, no. 3, pp. 245-255. https://doi.org/10.1093/intimm/dxn144
De Maria, Andrea ; Ugolotti, Elisabetta ; Rutjens, Erik ; Mazza, Stefania ; Radic, Luana ; Faravelli, Alessandro ; Koopman, Gerrit ; Di Marco, Eddi ; Costa, Paola ; Ensoli, Barbara ; Cafaro, Aurelio ; Mingari, Maria Cristina ; Moretta, Lorenzo ; Heeney, Jonathan ; Biassoni, Roberto. / NKp44 expression, phylogenesis and function in non-human primate NK cells. In: International Immunology. 2009 ; Vol. 21, No. 3. pp. 245-255.
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AU - Faravelli, Alessandro

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AU - Di Marco, Eddi

AU - Costa, Paola

AU - Ensoli, Barbara

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N2 - Molecular and functional characterization of the natural cytotoxicity receptor (NCR) NKp44 in species other than Homo sapiens has been elusive, so far. Here, we provide complete phenotypic, molecular and functional characterization for NKp44 triggering receptor on Pan troglodytes NK cells, the closest human relative, and the analysis of NKp44-genomic locus and transcription in Macaca fascicularis. Similar to H. sapiens, NKp44 expression is detectable on chimpanzee NK cells only upon activation. However, basal NKp44 transcription is 5-fold higher in chimpanzees with lower differential increases upon cell activation compared with humans. Upon activation, an overall 12-fold lower NKp44 gene expression is observed in P. troglodytes compared with H. sapiens NK cells with only a slight reduction in NKp44 surface expression. Functional analysis of 'in vitro' activated purified NK cells confirms the NKp44 triggering potential compared with other major NCRs. These findings suggest the presence of a post-transcriptional regulation that evolved differently in H. sapiens. Analysis of cynomolgus NKp44-genomic sequence and transcription pattern showed very low levels of transcription with occurrence of out-of-frame transcripts and no surface expression. The present comparative analysis suggests that NKp44-genomic organization appears during macaque speciation, with considerable evolution of its transcriptional and post-transcriptional tuning. Thus, NKp44 may represent an NCR being only recently emerged during speciation, acquiring functional relevance only in non-human primates closest to H. sapiens.

AB - Molecular and functional characterization of the natural cytotoxicity receptor (NCR) NKp44 in species other than Homo sapiens has been elusive, so far. Here, we provide complete phenotypic, molecular and functional characterization for NKp44 triggering receptor on Pan troglodytes NK cells, the closest human relative, and the analysis of NKp44-genomic locus and transcription in Macaca fascicularis. Similar to H. sapiens, NKp44 expression is detectable on chimpanzee NK cells only upon activation. However, basal NKp44 transcription is 5-fold higher in chimpanzees with lower differential increases upon cell activation compared with humans. Upon activation, an overall 12-fold lower NKp44 gene expression is observed in P. troglodytes compared with H. sapiens NK cells with only a slight reduction in NKp44 surface expression. Functional analysis of 'in vitro' activated purified NK cells confirms the NKp44 triggering potential compared with other major NCRs. These findings suggest the presence of a post-transcriptional regulation that evolved differently in H. sapiens. Analysis of cynomolgus NKp44-genomic sequence and transcription pattern showed very low levels of transcription with occurrence of out-of-frame transcripts and no surface expression. The present comparative analysis suggests that NKp44-genomic organization appears during macaque speciation, with considerable evolution of its transcriptional and post-transcriptional tuning. Thus, NKp44 may represent an NCR being only recently emerged during speciation, acquiring functional relevance only in non-human primates closest to H. sapiens.

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