NKRP1A molecule is involved in transendothelial migration of CD4+ human T lymphocytes

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23 Citations (Scopus)

Abstract

Among human CD4+ T lymphocytes, 5-20% express the C-type lectin molecule NKRP1A. Interestingly, CD4+ NKRP1A+ T lymphocytes express high levels of β1 and β2 integrins, thus representing a T lymphocyte subset that can possibly adhere and migrate through vascular endothelium. Indeed, resting CD4+ NKRP1A+ lymphocytes, differently from the CD4+ NKRP1A- subset, migrated across endothelial cell monolayers in a Transwell chamber system. This transendothelial migration was strongly reduced after pre-treatment with an anti-NKRP1A monoclonal antibody (mAb). In addition, the NKRP1A negative Jurkatt CD4+ T-cell line that had been stably transfected with NKRP1A cDNA, migrated more rapidly and efficiently than untransfected Jurkatt cells. Finally, mAb-mediated cross-linking of NKRP1A molecule in CD4+ T lymphocytes induced the upregulation of the LFA1 Mg2+ binding site as well as β1 and β2 integrin chains. Altogether, these findings indicate that NKRP1A molecule is involved in transendothelial migration of resting CD4+ T lymphocytes.

Original languageEnglish
Pages (from-to)121-123
Number of pages3
JournalImmunology Letters
Volume57
Issue number1-3
DOIs
Publication statusPublished - 1997

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Transendothelial and Transepithelial Migration
T-Lymphocytes
Integrins
Monoclonal Antibodies
C-Type Lectins
CD4 Antigens
Vascular Endothelium
T-Lymphocyte Subsets
Up-Regulation
Endothelial Cells
Complementary DNA
Binding Sites
Lymphocytes
Cell Line

Keywords

  • Integrins
  • Lectins
  • Lymphocyte migration
  • NKRP1A

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

NKRP1A molecule is involved in transendothelial migration of CD4+ human T lymphocytes. / Poggi, Alessandro; Costa, Paola; Zocchi, Maria Raffaella; Moretta, Lorenzo.

In: Immunology Letters, Vol. 57, No. 1-3, 1997, p. 121-123.

Research output: Contribution to journalArticle

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AU - Costa, Paola

AU - Zocchi, Maria Raffaella

AU - Moretta, Lorenzo

PY - 1997

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AB - Among human CD4+ T lymphocytes, 5-20% express the C-type lectin molecule NKRP1A. Interestingly, CD4+ NKRP1A+ T lymphocytes express high levels of β1 and β2 integrins, thus representing a T lymphocyte subset that can possibly adhere and migrate through vascular endothelium. Indeed, resting CD4+ NKRP1A+ lymphocytes, differently from the CD4+ NKRP1A- subset, migrated across endothelial cell monolayers in a Transwell chamber system. This transendothelial migration was strongly reduced after pre-treatment with an anti-NKRP1A monoclonal antibody (mAb). In addition, the NKRP1A negative Jurkatt CD4+ T-cell line that had been stably transfected with NKRP1A cDNA, migrated more rapidly and efficiently than untransfected Jurkatt cells. Finally, mAb-mediated cross-linking of NKRP1A molecule in CD4+ T lymphocytes induced the upregulation of the LFA1 Mg2+ binding site as well as β1 and β2 integrin chains. Altogether, these findings indicate that NKRP1A molecule is involved in transendothelial migration of resting CD4+ T lymphocytes.

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