Nkx2-5+islet1+ mesenchymal precursors generate distinct spleen stromal cell subsets and participate in restoring stromal network integrity

Laura Castagnaro, Elisa Lenti, Sara Maruzzelli, Laura Spinardi, Edoardo Migliori, Diego Farinello, Giovanni Sitia, Zachary Harrelson, Sylvia M. Evans, Luca G. Guidotti, Richard P. Harvey, Andrea Brendolan

Research output: Contribution to journalArticle

Abstract

Secondary lymphoid organ stromal cells comprise different subsets whose origins remain unknown. Herein, we exploit a genetic lineage-tracing approach to show that splenic fibroblastic reticular cells (FRCs), follicular dendritic cells (FDCs), marginal reticular cells (MRCs), and mural cells, but not endothelial cells, originate from embryonic mesenchymal progenitors of the Nkx2-5+Islet1+ lineage. This lineage include embryonic mesenchymal cells with lymphoid tissue organizer (LTo) activity capable also of supporting ectopic lymphoid-like structures and a subset of resident spleen stromal cells that proliferate and regenerate the splenic stromal microenvironment following resolution of a viral infection. These findings identify progenitor cells that generate stromal diversity in spleen development and repair and suggest the existence of multipotent stromal progenitors in the adult spleen with regenerative capacity.

Original languageEnglish
Pages (from-to)782-791
Number of pages10
JournalImmunity
Volume38
Issue number4
DOIs
Publication statusPublished - Apr 18 2013

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

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