NMDA-dependent NGF mRNA expression by human astrocytoma cells is mediated by nitric oxide

Tiziana Persichini, Marco Colasanti, Vincenzo Mollace, Giuseppe NisticÒ, Maria T. Ramacci, Giuliana M. Lauro

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to investigate the role of NO- cGMP pathway in NMDA-induced NGF mRNA expression by T67 astrocytoma cells. Levels of nitrite, a breakdown product of NO, in supernatants of NMDA- treated astrocytoma cells were significantly higher compared with control cells, this effect being reversed by the specific NO synthase inhibitor L-NAME. Furthermore, NGF mRNA expression was induced by NMDA treatment, this effect being inhibited by pretreatingcells with L-NAME. Moreover, methylene blue, an inhibitor of NO biological activity at guanylate cyclase level, inhibited NMDA-induced NGF mRNA expression and this effect was reversed by dbt2-cGMP. These findings suggest that NO-cGMP pathway mediates the synthesis of NGF mRNA.

Original languageEnglish
Pages (from-to)2477-2480
Number of pages4
JournalNeuroReport
Volume5
Issue number18
Publication statusPublished - 1994

Keywords

  • Astrocytoma
  • Cyclic GMP
  • Nerve growth factor
  • Nitric oxide
  • NMDA
  • RT-PCR

ASJC Scopus subject areas

  • Neuroscience(all)

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