NMDA receptor subunits are phosphorylated by activation of neurotrophin receptors in PSD of rat spinal cord

M. Di Luca; F. Gardoni; A. Finardi; S. Pagliardini; F. Cattabeni; G. Battaglia; C. Missale

NMDA receptor subunits are phosphorylated by activation of neurotrophin receptors in PSD of rat spinal cord

M. Di Luca, F. Gardoni, A. Finardi, S. Pagliardini, F. Cattabeni, G. Battaglia, C. Missale

Fondazione IRCCS Istituto Neurologico "C. Besta"

Research output: Contribution to journal › Article › peer-review

Abstract

We have investigated the distribution of NMDA and neurotrophin receptor systems and their reciprocal interactions in post-synaptic densities (PSD) purified from spinal cord. NMDA receptor subunits, trkA and trkB, but not trkC, were present in spinal cord PSD. The incubation of PSD with BDNF and NGF induced the phosphorylation of NR2A and B subunits. This phosphorylation was counteracted by antibodies directed against the catalytic domain of trkA and trkB receptors and by genistein. These results suggest the existence of a previously unexplored cross-talk between neurotrophins and NMDA receptors in rat spinal cord neurons.

Original language	English
Pages (from-to)	1301-1305
Number of pages	5
Journal	NeuroReport
Volume	12
Issue number	6
Publication status	Published - May 8 2001

Keywords

Neurotrophin receptors
NMDA
Phosphorylation
Postsynaptic density
Spinal cord

ASJC Scopus subject areas

Neuroscience(all)

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T1 - NMDA receptor subunits are phosphorylated by activation of neurotrophin receptors in PSD of rat spinal cord

AU - Di Luca, M.

AU - Gardoni, F.

AU - Finardi, A.

AU - Pagliardini, S.

AU - Cattabeni, F.

AU - Battaglia, G.

AU - Missale, C.

PY - 2001/5/8

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N2 - We have investigated the distribution of NMDA and neurotrophin receptor systems and their reciprocal interactions in post-synaptic densities (PSD) purified from spinal cord. NMDA receptor subunits, trkA and trkB, but not trkC, were present in spinal cord PSD. The incubation of PSD with BDNF and NGF induced the phosphorylation of NR2A and B subunits. This phosphorylation was counteracted by antibodies directed against the catalytic domain of trkA and trkB receptors and by genistein. These results suggest the existence of a previously unexplored cross-talk between neurotrophins and NMDA receptors in rat spinal cord neurons.

AB - We have investigated the distribution of NMDA and neurotrophin receptor systems and their reciprocal interactions in post-synaptic densities (PSD) purified from spinal cord. NMDA receptor subunits, trkA and trkB, but not trkC, were present in spinal cord PSD. The incubation of PSD with BDNF and NGF induced the phosphorylation of NR2A and B subunits. This phosphorylation was counteracted by antibodies directed against the catalytic domain of trkA and trkB receptors and by genistein. These results suggest the existence of a previously unexplored cross-talk between neurotrophins and NMDA receptors in rat spinal cord neurons.

KW - Neurotrophin receptors

KW - NMDA

KW - Phosphorylation

KW - Postsynaptic density

KW - Spinal cord

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NMDA receptor subunits are phosphorylated by activation of neurotrophin receptors in PSD of rat spinal cord

Abstract

Keywords

ASJC Scopus subject areas

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