NMR analysis of low-density lipoprotein oxidatively-modified in vztro

L. Barenghi; S. Bradamante; G. A. Giudici; C. Verganl

doi:10.3109/10715769009087991

NMR analysis of low-density lipoprotein oxidatively-modified in vztro

L. Barenghi, S. Bradamante, G. A. Giudici, C. Verganl

Fondazione IRCCS Ca' Granda- Ospedale Maggiore Policlinico

Research output: Contribution to journal › Article › peer-review

Abstract

Human plasma low density lipoprotein has been oxidized at different stages in vitro and analysed by ¹H. ¹³C, and ³¹P NMR spectroscopy and by biochemical methods. Information was obtained on: a) structure mobilities of lipids and on lipid-protein interactions: b) conjugated and oxo-dienes; c) polyunsaturated/ monounsaturated fatty acid chains; d) lysophosphatidylcholine production. The results show that the NMR approach is particularly useful for the assessment of structural modification in oxidized LDL.

Original language	English
Pages (from-to)	175-183
Number of pages	9
Journal	Free Radical Research
Volume	8
Issue number	3
DOIs	https://doi.org/10.3109/10715769009087991
Publication status	Published - 1990

Keywords

Ageing
Atherosclerosis
Lipid peroxidation
NMR spectroscopy
Oxidatively-modified LDL

ASJC Scopus subject areas

Biochemistry

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abstract = "Human plasma low density lipoprotein has been oxidized at different stages in vitro and analysed by 1H. 13C, and 31P NMR spectroscopy and by biochemical methods. Information was obtained on: a) structure mobilities of lipids and on lipid-protein interactions: b) conjugated and oxo-dienes; c) polyunsaturated/ monounsaturated fatty acid chains; d) lysophosphatidylcholine production. The results show that the NMR approach is particularly useful for the assessment of structural modification in oxidized LDL.",

keywords = "Ageing, Atherosclerosis, Lipid peroxidation, NMR spectroscopy, Oxidatively-modified LDL",

author = "L. Barenghi and S. Bradamante and Giudici, {G. A.} and C. Verganl",

year = "1990",

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language = "English",

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journal = "Free Radical Research",

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AU - Barenghi, L.

AU - Bradamante, S.

AU - Giudici, G. A.

AU - Verganl, C.

PY - 1990

Y1 - 1990

N2 - Human plasma low density lipoprotein has been oxidized at different stages in vitro and analysed by 1H. 13C, and 31P NMR spectroscopy and by biochemical methods. Information was obtained on: a) structure mobilities of lipids and on lipid-protein interactions: b) conjugated and oxo-dienes; c) polyunsaturated/ monounsaturated fatty acid chains; d) lysophosphatidylcholine production. The results show that the NMR approach is particularly useful for the assessment of structural modification in oxidized LDL.

AB - Human plasma low density lipoprotein has been oxidized at different stages in vitro and analysed by 1H. 13C, and 31P NMR spectroscopy and by biochemical methods. Information was obtained on: a) structure mobilities of lipids and on lipid-protein interactions: b) conjugated and oxo-dienes; c) polyunsaturated/ monounsaturated fatty acid chains; d) lysophosphatidylcholine production. The results show that the NMR approach is particularly useful for the assessment of structural modification in oxidized LDL.

KW - Ageing

KW - Atherosclerosis

KW - Lipid peroxidation

KW - NMR spectroscopy

KW - Oxidatively-modified LDL

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