Abstract
Human plasma low density lipoprotein has been oxidized at different stages in vitro and analysed by 1H. 13C, and 31P NMR spectroscopy and by biochemical methods. Information was obtained on: a) structure mobilities of lipids and on lipid-protein interactions: b) conjugated and oxo-dienes; c) polyunsaturated/ monounsaturated fatty acid chains; d) lysophosphatidylcholine production. The results show that the NMR approach is particularly useful for the assessment of structural modification in oxidized LDL.
| Original language | English |
|---|---|
| Pages (from-to) | 175-183 |
| Number of pages | 9 |
| Journal | Free Radical Research |
| Volume | 8 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1990 |
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Keywords
- Ageing
- Atherosclerosis
- Lipid peroxidation
- NMR spectroscopy
- Oxidatively-modified LDL
ASJC Scopus subject areas
- Biochemistry
Cite this
NMR analysis of low-density lipoprotein oxidatively-modified in vztro. / Barenghi, L.; Bradamante, S.; Giudici, G. A.; Verganl, C.
In: Free Radical Research, Vol. 8, No. 3, 1990, p. 175-183.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - NMR analysis of low-density lipoprotein oxidatively-modified in vztro
AU - Barenghi, L.
AU - Bradamante, S.
AU - Giudici, G. A.
AU - Verganl, C.
PY - 1990
Y1 - 1990
N2 - Human plasma low density lipoprotein has been oxidized at different stages in vitro and analysed by 1H. 13C, and 31P NMR spectroscopy and by biochemical methods. Information was obtained on: a) structure mobilities of lipids and on lipid-protein interactions: b) conjugated and oxo-dienes; c) polyunsaturated/ monounsaturated fatty acid chains; d) lysophosphatidylcholine production. The results show that the NMR approach is particularly useful for the assessment of structural modification in oxidized LDL.
AB - Human plasma low density lipoprotein has been oxidized at different stages in vitro and analysed by 1H. 13C, and 31P NMR spectroscopy and by biochemical methods. Information was obtained on: a) structure mobilities of lipids and on lipid-protein interactions: b) conjugated and oxo-dienes; c) polyunsaturated/ monounsaturated fatty acid chains; d) lysophosphatidylcholine production. The results show that the NMR approach is particularly useful for the assessment of structural modification in oxidized LDL.
KW - Ageing
KW - Atherosclerosis
KW - Lipid peroxidation
KW - NMR spectroscopy
KW - Oxidatively-modified LDL
UR - http://www.scopus.com/inward/record.url?scp=0025236648&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025236648&partnerID=8YFLogxK
U2 - 10.3109/10715769009087991
DO - 10.3109/10715769009087991
M3 - Article
C2 - 2328928
AN - SCOPUS:0025236648
VL - 8
SP - 175
EP - 183
JO - Free Radical Research
JF - Free Radical Research
SN - 1071-5762
IS - 3
ER -