NMR analysis of low-density lipoprotein oxidatively-modified in vztro

L. Barenghi, S. Bradamante, G. A. Giudici, C. Verganl

Research output: Contribution to journalArticle

Abstract

Human plasma low density lipoprotein has been oxidized at different stages in vitro and analysed by 1H. 13C, and 31P NMR spectroscopy and by biochemical methods. Information was obtained on: a) structure mobilities of lipids and on lipid-protein interactions: b) conjugated and oxo-dienes; c) polyunsaturated/ monounsaturated fatty acid chains; d) lysophosphatidylcholine production. The results show that the NMR approach is particularly useful for the assessment of structural modification in oxidized LDL.

Original languageEnglish
Pages (from-to)175-183
Number of pages9
JournalFree Radical Research
Volume8
Issue number3
DOIs
Publication statusPublished - 1990

Fingerprint

LDL Lipoproteins
Nuclear magnetic resonance
Polyunsaturated fatty acids
Plasma (human)
Lipids
Monounsaturated Fatty Acids
Lysophosphatidylcholines
Unsaturated Fatty Acids
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Proteins
oxidized low density lipoprotein
In Vitro Techniques
Carbon-13 Magnetic Resonance Spectroscopy

Keywords

  • Ageing
  • Atherosclerosis
  • Lipid peroxidation
  • NMR spectroscopy
  • Oxidatively-modified LDL

ASJC Scopus subject areas

  • Biochemistry

Cite this

NMR analysis of low-density lipoprotein oxidatively-modified in vztro. / Barenghi, L.; Bradamante, S.; Giudici, G. A.; Verganl, C.

In: Free Radical Research, Vol. 8, No. 3, 1990, p. 175-183.

Research output: Contribution to journalArticle

Barenghi, L, Bradamante, S, Giudici, GA & Verganl, C 1990, 'NMR analysis of low-density lipoprotein oxidatively-modified in vztro', Free Radical Research, vol. 8, no. 3, pp. 175-183. https://doi.org/10.3109/10715769009087991
Barenghi, L. ; Bradamante, S. ; Giudici, G. A. ; Verganl, C. / NMR analysis of low-density lipoprotein oxidatively-modified in vztro. In: Free Radical Research. 1990 ; Vol. 8, No. 3. pp. 175-183.
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