NMR as evaluation strategy for cellular uptake of nanoparticles

Tomas Orlando, Alessandro Paolini, Francesco Pineider, Emanuela Clementi, Francesca Pasi, Yannick Guari, Joulia Larionova, Luciano Sacchi, Rosanna Nano, Maurizio Corti, Alessandro Lascialfari

Research output: Contribution to journalArticle

Abstract

Advanced nanostructured materials, such as gold nanoparticles, magnetic nanoparticles, and multifunctional materials, are nowadays used in many state-of-the-art biomedical application. However, although the engineering in this field is very advanced, there remain some fundamental problems involving the interaction mechanisms between nanostructures and cells or tissues. Here we show the potential of 1H NMR in the investigation of the uptake of two different kinds of nanostructures, that is, maghemite and gold nanoparticles, and of a chemotherapy drug (Temozolomide) in glioblastoma tumor cells. The proposed experimental protocol provides a new way to investigate the general problem of cellular uptake for a variety of biocompatible nanostructures and drugs.

Original languageEnglish
Pages (from-to)3959-3965
Number of pages7
JournalNano Letters
Volume14
Issue number7
DOIs
Publication statusPublished - Jul 9 2014

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Keywords

  • cellular response
  • cellular uptake
  • gold nanoparticles
  • Magnetic nanoparticles

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering
  • Medicine(all)

Cite this

Orlando, T., Paolini, A., Pineider, F., Clementi, E., Pasi, F., Guari, Y., Larionova, J., Sacchi, L., Nano, R., Corti, M., & Lascialfari, A. (2014). NMR as evaluation strategy for cellular uptake of nanoparticles. Nano Letters, 14(7), 3959-3965. https://doi.org/10.1021/nl501282x