NMR relaxometric investigation on human methemoglobin and fluoromethemoglobin. An improved quantitative in vitro assay of human methemoglobin

S. Aime, M. Fasano, S. Paoletti, A. Arnelli, P. Ascenzi

Research output: Contribution to journalArticlepeer-review

Abstract

Longitudinal water proton relaxation rates of methemoglobin solutions show a strong dependence on temperature and pH. The increase of the relaxation rates with temperature is associated with shortened exchange lifetime of the coordinated water molecule. An accurate measurement of the relaxation rate of methemoglobin solutions thus requires careful control of the experimental temperature. This observation prompted the authors to look for an improved version of the relaxometric in vitro determination of methemoglobin. The method is based on transforming methemoglobin into the corresponding fluoromethemoglobin derivative, which shows both a higher relaxivity and a negligible dependence on temperature. The proposed method has been found to be in good agreement with data from spectrophotometric assays.

Original languageEnglish
Pages (from-to)827-831
Number of pages5
JournalMagnetic Resonance in Medicine
Volume33
Issue number6
DOIs
Publication statusPublished - 1995

Keywords

  • fluoromethemoglobin
  • interlaboratory measurements
  • methemoglobin assay
  • NMR relaxometry

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

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