NMR structure of the p63 SAM domain and dynamical properties of G534V and T537P pathological mutants, identified in the AEC syndrome

Daniel O. Cicero, Mattia Falconi, Eleonora Candi, Sonia Mele, Bruno Cadot, Almerinda Di Venere, Stefano Rufini, Gerry Melino, Alessandro Desideri

Research output: Contribution to journalArticlepeer-review

Abstract

The p63 protein is crucial for epidermal development, and its mutations cause the extrodactyly ectodermal dysplasia and cleft lip/palate syndrome. The three-dimensional solution structure of the p63 sterile α-motif (SAM) domain (residues 505-579), a region crucial to explaining the human genetic disease ankyloblepharonectodermal dysplasia-clefting syndrome (AEC), has been determined by nuclear magnetic resonance spectroscopy The structure indicates that the domain is a monomer with the characteristic five-helix bundle topology observed in other SAM domains. It includes five tightly packed helices with an extended hydrophobic core to form a globular and compact structure. The dynamics of the backbone and the global correlation time of the molecule have also been investigated and compared with the dynamical properties obtained through molecular dynamics simulation. Attempts to purify the pathological G534V and T537P mutants, originally identified in AEC, were not successful because of the occurrence of unspecific proteolytic degradation of the mutated SAM domains. Analysis of the structural dynamic properties of the G534V and T537P mutants through molecular dynamics simulation and comparison with the wild type permits detection of differences in the degree of freedom of individual residues and discussion of the possible causes for the pathology.

Original languageEnglish
Pages (from-to)475-489
Number of pages15
JournalCell Biochemistry and Biophysics
Volume44
Issue number3
DOIs
Publication statusPublished - 2006

Keywords

  • Ankyloblepharon-ectodermal dysplasia-clefting syndrome
  • Ectodermal dysplasia and cleft lip/palate syndrome
  • Molecular dynamics
  • NMR
  • p53 family
  • p63
  • Structure
  • Structure destabilization

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Biophysics
  • Biochemistry

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