No detection of varicella-zoster virus in temporal arteries of patients with giant cell arteritis

Francesco Muratore, Stefania Croci, Ione Tamagnini, Alessandro Zerbini, Salvatore Bellafiore, Lucia Belloni, Luigi Boiardi, Alessandra Bisagni, Nicolò Pipitone, Maria Parmeggiani, Alberto Cavazza, Carlo Salvarani

Research output: Contribution to journalArticle

Abstract

Objective: Data on the presence of varicella-zoster virus (VZV) in temporal arteries of patients with giant cell arteritis (GCA) are controversial. We analyzed VZV infection in temporal arteries from Italian patients with temporal artery biopsy (TAB)-positive GCA, TAB-negative GCA, and controls. Methods: A total of 79 formalin-fixed, paraffin-embedded (FFPE) TABs performed between 2009 and 2012 at a single institution from 34 TAB-positive GCA patients, 15 TAB-negative GCA patients, and 30 controls were retrieved. Six 5-μm sections of all FFPE TABs were cut. The first section was analyzed by immunohistochemistry using mouse monoclonal anti-VZVgE IgG1 antibody. DNA was extracted from the remaining five sections and analyzed by real-time polymerase chain reaction (PCR) for the presence of VZV DNA. For 10 of the 34 TAB-positive GCA patients, an additional 2-mm piece of frozen TAB was available. DNA was extracted from the entire 2-mm length frozen specimen and analyzed by PCR for the presence of VZV DNA. Thirty additional 5-μm sections were cut from the FFPE TABs of these 10 patients and analyzed by immunohistochemistry for the presence of VZV antigen. Results: Immunohistochemical analysis detected VZV antigen in 1/34 (3%) TAB-positive GCA, 0/15 TAB-negative GCA, and 0/30 controls, and in none of the 300 sections cut from the 10 FFPE TABs positive for GCA for which the frozen specimens were available. DNA obtained from all TABs was amplifiable. VZV DNA was neither found in any of the FFPE TABs nor found in frozen TABs. Conclusion: Our data do not support in Italian patients a possible role for VZV infection in the etiopathogenesis of GCA.

Original languageEnglish
JournalSeminars in Arthritis and Rheumatism
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Temporal Arteries
Giant Cell Arteritis
Human Herpesvirus 3
Biopsy
Paraffin
Formaldehyde
DNA
Virus Diseases
Immunohistochemistry
Antigens
Real-Time Polymerase Chain Reaction
Immunoglobulin G

Keywords

  • Giant cell arteritis
  • Infection
  • Temporal arteritis
  • Temporal artery biopsy
  • Varicella-zoster virus

ASJC Scopus subject areas

  • Rheumatology
  • Anesthesiology and Pain Medicine

Cite this

No detection of varicella-zoster virus in temporal arteries of patients with giant cell arteritis. / Muratore, Francesco; Croci, Stefania; Tamagnini, Ione; Zerbini, Alessandro; Bellafiore, Salvatore; Belloni, Lucia; Boiardi, Luigi; Bisagni, Alessandra; Pipitone, Nicolò; Parmeggiani, Maria; Cavazza, Alberto; Salvarani, Carlo.

In: Seminars in Arthritis and Rheumatism, 2017.

Research output: Contribution to journalArticle

Muratore, F, Croci, S, Tamagnini, I, Zerbini, A, Bellafiore, S, Belloni, L, Boiardi, L, Bisagni, A, Pipitone, N, Parmeggiani, M, Cavazza, A & Salvarani, C 2017, 'No detection of varicella-zoster virus in temporal arteries of patients with giant cell arteritis', Seminars in Arthritis and Rheumatism. https://doi.org/10.1016/j.semarthrit.2017.02.005
Muratore, Francesco ; Croci, Stefania ; Tamagnini, Ione ; Zerbini, Alessandro ; Bellafiore, Salvatore ; Belloni, Lucia ; Boiardi, Luigi ; Bisagni, Alessandra ; Pipitone, Nicolò ; Parmeggiani, Maria ; Cavazza, Alberto ; Salvarani, Carlo. / No detection of varicella-zoster virus in temporal arteries of patients with giant cell arteritis. In: Seminars in Arthritis and Rheumatism. 2017.
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abstract = "Objective: Data on the presence of varicella-zoster virus (VZV) in temporal arteries of patients with giant cell arteritis (GCA) are controversial. We analyzed VZV infection in temporal arteries from Italian patients with temporal artery biopsy (TAB)-positive GCA, TAB-negative GCA, and controls. Methods: A total of 79 formalin-fixed, paraffin-embedded (FFPE) TABs performed between 2009 and 2012 at a single institution from 34 TAB-positive GCA patients, 15 TAB-negative GCA patients, and 30 controls were retrieved. Six 5-μm sections of all FFPE TABs were cut. The first section was analyzed by immunohistochemistry using mouse monoclonal anti-VZVgE IgG1 antibody. DNA was extracted from the remaining five sections and analyzed by real-time polymerase chain reaction (PCR) for the presence of VZV DNA. For 10 of the 34 TAB-positive GCA patients, an additional 2-mm piece of frozen TAB was available. DNA was extracted from the entire 2-mm length frozen specimen and analyzed by PCR for the presence of VZV DNA. Thirty additional 5-μm sections were cut from the FFPE TABs of these 10 patients and analyzed by immunohistochemistry for the presence of VZV antigen. Results: Immunohistochemical analysis detected VZV antigen in 1/34 (3{\%}) TAB-positive GCA, 0/15 TAB-negative GCA, and 0/30 controls, and in none of the 300 sections cut from the 10 FFPE TABs positive for GCA for which the frozen specimens were available. DNA obtained from all TABs was amplifiable. VZV DNA was neither found in any of the FFPE TABs nor found in frozen TABs. Conclusion: Our data do not support in Italian patients a possible role for VZV infection in the etiopathogenesis of GCA.",
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T1 - No detection of varicella-zoster virus in temporal arteries of patients with giant cell arteritis

AU - Muratore, Francesco

AU - Croci, Stefania

AU - Tamagnini, Ione

AU - Zerbini, Alessandro

AU - Bellafiore, Salvatore

AU - Belloni, Lucia

AU - Boiardi, Luigi

AU - Bisagni, Alessandra

AU - Pipitone, Nicolò

AU - Parmeggiani, Maria

AU - Cavazza, Alberto

AU - Salvarani, Carlo

PY - 2017

Y1 - 2017

N2 - Objective: Data on the presence of varicella-zoster virus (VZV) in temporal arteries of patients with giant cell arteritis (GCA) are controversial. We analyzed VZV infection in temporal arteries from Italian patients with temporal artery biopsy (TAB)-positive GCA, TAB-negative GCA, and controls. Methods: A total of 79 formalin-fixed, paraffin-embedded (FFPE) TABs performed between 2009 and 2012 at a single institution from 34 TAB-positive GCA patients, 15 TAB-negative GCA patients, and 30 controls were retrieved. Six 5-μm sections of all FFPE TABs were cut. The first section was analyzed by immunohistochemistry using mouse monoclonal anti-VZVgE IgG1 antibody. DNA was extracted from the remaining five sections and analyzed by real-time polymerase chain reaction (PCR) for the presence of VZV DNA. For 10 of the 34 TAB-positive GCA patients, an additional 2-mm piece of frozen TAB was available. DNA was extracted from the entire 2-mm length frozen specimen and analyzed by PCR for the presence of VZV DNA. Thirty additional 5-μm sections were cut from the FFPE TABs of these 10 patients and analyzed by immunohistochemistry for the presence of VZV antigen. Results: Immunohistochemical analysis detected VZV antigen in 1/34 (3%) TAB-positive GCA, 0/15 TAB-negative GCA, and 0/30 controls, and in none of the 300 sections cut from the 10 FFPE TABs positive for GCA for which the frozen specimens were available. DNA obtained from all TABs was amplifiable. VZV DNA was neither found in any of the FFPE TABs nor found in frozen TABs. Conclusion: Our data do not support in Italian patients a possible role for VZV infection in the etiopathogenesis of GCA.

AB - Objective: Data on the presence of varicella-zoster virus (VZV) in temporal arteries of patients with giant cell arteritis (GCA) are controversial. We analyzed VZV infection in temporal arteries from Italian patients with temporal artery biopsy (TAB)-positive GCA, TAB-negative GCA, and controls. Methods: A total of 79 formalin-fixed, paraffin-embedded (FFPE) TABs performed between 2009 and 2012 at a single institution from 34 TAB-positive GCA patients, 15 TAB-negative GCA patients, and 30 controls were retrieved. Six 5-μm sections of all FFPE TABs were cut. The first section was analyzed by immunohistochemistry using mouse monoclonal anti-VZVgE IgG1 antibody. DNA was extracted from the remaining five sections and analyzed by real-time polymerase chain reaction (PCR) for the presence of VZV DNA. For 10 of the 34 TAB-positive GCA patients, an additional 2-mm piece of frozen TAB was available. DNA was extracted from the entire 2-mm length frozen specimen and analyzed by PCR for the presence of VZV DNA. Thirty additional 5-μm sections were cut from the FFPE TABs of these 10 patients and analyzed by immunohistochemistry for the presence of VZV antigen. Results: Immunohistochemical analysis detected VZV antigen in 1/34 (3%) TAB-positive GCA, 0/15 TAB-negative GCA, and 0/30 controls, and in none of the 300 sections cut from the 10 FFPE TABs positive for GCA for which the frozen specimens were available. DNA obtained from all TABs was amplifiable. VZV DNA was neither found in any of the FFPE TABs nor found in frozen TABs. Conclusion: Our data do not support in Italian patients a possible role for VZV infection in the etiopathogenesis of GCA.

KW - Giant cell arteritis

KW - Infection

KW - Temporal arteritis

KW - Temporal artery biopsy

KW - Varicella-zoster virus

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