Nomifensine but not amantadine increases dopamine-induced responses on rat substantia nigra zona compacta neurons

Nicola B. Mercuri, Francesca Stratta, Paolo Calabresi, Giorgio Bernardi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Responses of substantia nigra zona compacta neurons to nomifensine and amantadine were studied with intracellular recording techniques (current and voltage clamp) in in vitro slice preparation of rat mesencephalon. The application of nomifensine (1-10 μM) slightly hyperpolarized the cells and inhibited action potential discharge that occurs spontaneously. In voltage-clamp experiments (-50, -60 mV, holding potential) an outward current was observed. The membrane responses to exogenously-applied dopamine were potentiated by the concomitant superfusion of nomifensine. The effects of nomifensine were antagonized by (-)-sulpiride (1 μM), a D2 receptor antagonist. By contrast, the superfusion of amantadine (1-30 μM) on substantia nigra zona compacta cells was ineffective on firing rate, membrane potential or on sensitivity to exogenous dopamine. In the presence of high doses (300 μM to 1 mM) of amantadine a depolarization and an increase in firing activity was observed. While our results provide electrophysiological evidence for an inhibition of the dopamine uptake system by nomifensine, they do not support a dopaminergic mechanism for the actions of amantadine in the substantia nigra zona compacta.

Original languageEnglish
Pages (from-to)145-148
Number of pages4
JournalNeuroscience Letters
Volume131
Issue number2
DOIs
Publication statusPublished - Oct 14 1991

Fingerprint

Nomifensine
Amantadine
Herpes Zoster
Dopamine
Neurons
Sulpiride
Patch-Clamp Techniques
Mesencephalon
Membrane Potentials
Action Potentials
Pars Compacta
Membranes

Keywords

  • Amantadine
  • Current clamp
  • DA uptake
  • Depression
  • Dopamine
  • Nomifensine
  • Parkinson's disease
  • Voltage clamp

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Nomifensine but not amantadine increases dopamine-induced responses on rat substantia nigra zona compacta neurons. / Mercuri, Nicola B.; Stratta, Francesca; Calabresi, Paolo; Bernardi, Giorgio.

In: Neuroscience Letters, Vol. 131, No. 2, 14.10.1991, p. 145-148.

Research output: Contribution to journalArticle

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