Non-invasive tissue diagnostics using a multimodal spectroscopic device based on fiber probe

Riccardo Cicchi, Suresh Anand, Susanna Rossari, Alessandro Sturiale, Flavio Giordano, Vincenzo De Giorgi, Vincenza Maio, Daniela Massi, Gabriella Nesi, Anna Maria Buccoliero, Francesco Tonelli, Renzo Guerrini, Nicola Pimpinelli, Francesco Saverio Pavone

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Two different optical fiber probes for combined Raman and fluorescence spectroscopic measurements were designed, developed and used for tissue diagnostics. Two visible laser diodes were used for fluorescence spectroscopy, whereas a laser diode emitting in the NIR was used for Raman spectroscopy. The two probes were based on fiber bundles with a central multimode optical fiber, used for delivering light to the tissue, and 24 surrounding optical fibers for signal collection. Both fluorescence and Raman spectra were acquired using the same detection unit, based on a cooled CCD camera, connected to a spectrograph. The two probes were successfully employed for diagnosing melanocytic lesions in a good agreement with common routine histology. The obtained results demonstrated that the multimodal approach is crucial for improving diagnostic capabilities. Further investigations were performed on colon and brain tissue samples in order to have a benchmark for diagnosing a broader range of tissue lesions and malignancies. The system presented here can improve diagnostic capabilities on a broad range of tissues and has the potential of being used for endoscopic inspections in the near future.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSPIE
Volume9129
ISBN (Print)9781628410778
DOIs
Publication statusPublished - 2014
EventBiophotonics: Photonic Solutions for Better Health Care IV - Brussels, Belgium
Duration: Apr 14 2014Apr 17 2014

Other

OtherBiophotonics: Photonic Solutions for Better Health Care IV
CountryBelgium
CityBrussels
Period4/14/144/17/14

Fingerprint

Optical Fiber
Diagnostics
Probe
Laser Diode
Fiber
Tissue
Fluorescence
fibers
Fibers
probes
Optical fibers
optical fibers
Fluorescence Spectroscopy
Histology
Multimode Fiber
Raman Spectra
Raman Spectroscopy
fluorescence
lesions
Fiber Bundle

Keywords

  • (170.6935)Tissue characterization
  • (300.6280) Spectroscopy
  • (300.6450) Spectroscopy
  • fluorescence and luminescence
  • Raman

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Cicchi, R., Anand, S., Rossari, S., Sturiale, A., Giordano, F., De Giorgi, V., ... Pavone, F. S. (2014). Non-invasive tissue diagnostics using a multimodal spectroscopic device based on fiber probe. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 9129). [912932] SPIE. https://doi.org/10.1117/12.2052197

Non-invasive tissue diagnostics using a multimodal spectroscopic device based on fiber probe. / Cicchi, Riccardo; Anand, Suresh; Rossari, Susanna; Sturiale, Alessandro; Giordano, Flavio; De Giorgi, Vincenzo; Maio, Vincenza; Massi, Daniela; Nesi, Gabriella; Buccoliero, Anna Maria; Tonelli, Francesco; Guerrini, Renzo; Pimpinelli, Nicola; Pavone, Francesco Saverio.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 9129 SPIE, 2014. 912932.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Cicchi, R, Anand, S, Rossari, S, Sturiale, A, Giordano, F, De Giorgi, V, Maio, V, Massi, D, Nesi, G, Buccoliero, AM, Tonelli, F, Guerrini, R, Pimpinelli, N & Pavone, FS 2014, Non-invasive tissue diagnostics using a multimodal spectroscopic device based on fiber probe. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 9129, 912932, SPIE, Biophotonics: Photonic Solutions for Better Health Care IV, Brussels, Belgium, 4/14/14. https://doi.org/10.1117/12.2052197
Cicchi R, Anand S, Rossari S, Sturiale A, Giordano F, De Giorgi V et al. Non-invasive tissue diagnostics using a multimodal spectroscopic device based on fiber probe. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 9129. SPIE. 2014. 912932 https://doi.org/10.1117/12.2052197
Cicchi, Riccardo ; Anand, Suresh ; Rossari, Susanna ; Sturiale, Alessandro ; Giordano, Flavio ; De Giorgi, Vincenzo ; Maio, Vincenza ; Massi, Daniela ; Nesi, Gabriella ; Buccoliero, Anna Maria ; Tonelli, Francesco ; Guerrini, Renzo ; Pimpinelli, Nicola ; Pavone, Francesco Saverio. / Non-invasive tissue diagnostics using a multimodal spectroscopic device based on fiber probe. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 9129 SPIE, 2014.
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