Non-MHC-restricted target-cell lysis by a CD4-CD8- TCRαβ T-cell line, as well as by TCRγδ T-cell lines, results from lymphokine-activated killing

R. T. Maziarz, V. Groh, M. Predergast, M. Fabbi, J. L. Strominger, S. J. Burakoff

Research output: Contribution to journalArticlepeer-review

Abstract

A long-term CD4-CD8- TCRαβ human T-cell line, as well as similar CD4-CD8- TCRγδ T-cell lines for comparison, were generated from various tissues by negative selection using anti-CD4 and anti-CD8 monoclonal antibodies (MAbs) followed by positive selection with specific anti-TCR MAb and then repeated in vitro stimulation with interleukin-enriched media and lectin. These cell lines all demonstrated non-MHC-restricted cytolysis on a variety of human tumor cell lines. However, removal of lymphokines form the culture media for 24 hr abrogated most of the non-MHC-restricted target-cell lysis without affecting TCRαβ or TCRγδ cell viability of TCR function as determined by antibody-triggered redirected target-cell lysis. Subsequent re-exposure to lymphokines reconstituted non-MHC-restricted cytolysis by these cell lines. Thus, much of the non-specific, non-MHC-restricted cytolytic activity generated by CD4-CD8- TCRαβ or TCRγδ cells is secondary to lymphokine-activated killing (LAK) activity. These cells have potent LAK activity and may be prominent in LAK-cell populations. In addition, after lymphokine deprivation, both CD4-CD8- TCRαβ and TCRγδ cells showed residual activity against some tumor-cell targets, the nature of which remains to be defined.

Original languageEnglish
Pages (from-to)142-147
Number of pages6
JournalInternational Journal of Cancer
Volume48
Issue number1
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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