Abstract
A comprehensive overview is given concerning the in situ hybridization techniques using non-radioactively labelled probes. Details are given on the preparation of the probes with different reporter molecules and on the corresponding revealing assays. The development of non-isotopic labelling methods for nucleic acid probes has facilitated the diffusion of the in situ hybridization techniques to the research as well as to the clinical pathology laboratory. Non-radioactive probes have several advantages over the radiolabelled ones, including cost, shelf-life, safety and, last but not least, resolution of the signal.
Original language | English |
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Pages (from-to) | 243-251 |
Number of pages | 9 |
Journal | Liver |
Volume | 12 |
Issue number | 4 II |
Publication status | Published - 1992 |
ASJC Scopus subject areas
- Hepatology