Non-random chromosomal distribution of SSLPs: Systematic assessment using a novel genetic linkage map between two closely related rat strains

B. Gigante, S. Rubattu, R. Y L Zee, M. Volpe, Klaus Lindpaintner

Research output: Contribution to journalArticle

Abstract

Simple sequence length polymorphisms (SSLPs) are a widely used tool for genetic studies in humans and model animals. Experimental crosses among closely related strains that differ primarily in the trait that is to be mapped carry the advantage of avoiding co-segregation of potentially confounding traits. However, their realization is encumbered by the limited availability of newly arisen informative SSLPs among such strains. Here we report the establishment of a genome-wide SSLP panel for the spontaneously hypertensive rat (SHR) and its close relative, the stroke-prone SHR (SHRSP), consisting of a total of 273 polymorphic markers that were found among 2,734 rat SSLPs screened. In addition to limitations in numbers, we also found the distribution of informative markers to be heterogeneous, with clustering and paucity of informative markers, respectively, in particular regions. Notably, the majority of regions thus identified was also seen when we examined an unrelated set of strains from the literature, indicating, on a more generic level, the presence of mutagenically more and less stable genomic regions.

Original languageEnglish
Pages (from-to)64-72
Number of pages9
JournalCytogenetics and Cell Genetics
Volume95
Issue number1-2
Publication statusPublished - 2001

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Genetic Linkage
Inbred SHR Rats
Cluster Analysis
Animal Models
Stroke
Genome

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Non-random chromosomal distribution of SSLPs : Systematic assessment using a novel genetic linkage map between two closely related rat strains. / Gigante, B.; Rubattu, S.; Zee, R. Y L; Volpe, M.; Lindpaintner, Klaus.

In: Cytogenetics and Cell Genetics, Vol. 95, No. 1-2, 2001, p. 64-72.

Research output: Contribution to journalArticle

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