Non specific cytotoxicity of spleen cells in mice bearing transplanted chemically induced fibrosarcomas

A. Mantovani, R. Evans, P. Alexander

Research output: Contribution to journalArticlepeer-review

Abstract

Spleen cells collected from mice bearing transplanted chemically induced syngeneic fibrosarcomas non-specifically inhibited DNA synthesis of sarcoma and lymphoma target cells in vitro. Splenocytes from mice hyper-immunized against a syngeneic sarcoma specifically inhibited DNA synthesis of the tumor used for immunization. The impairment of tumor-cell DNA synthesis was associated in vitro with cytostasis, and lysis of the target cells was not seen. Since treatment with anti-theta serum and complement did not impair cytostatic action of the spleen cells, and since thymus-deprived animals showed similar activity to normal mice, T lymphocytes were not involved in non-specific cytostasis. Removal of phagocytic adherent cells by carbonyl iron markedly inhibited the cytostatic activity of the spleen cells, suggesting a role in this reaction for cells of the monocyte-macrophage series. The presence of an actively growing sarcoma was a prerequisite for the expression of non-specific cytostasis, since surgical excision resulted in complete disappearance of this activity of spleen cells.

Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalBritish Journal of Cancer
Volume36
Issue number1
Publication statusPublished - 1977

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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