Non-virally mediated gene transfer into human central nervous system precursor cells

Elena Cattaneo, Luciano Conti, Angela Gritti, Paola Frolichsthal, Stefano Govoni, Angelo Vescovi

Research output: Contribution to journalArticlepeer-review

Abstract

Lipofectamine-based transfection was used as a method of choice to deliver the bacterial β-galactosidase gene into human central nervous system (CNS) precursor cells. We achieved a transfection efficiency of 7.4%. β-Galactosidase expressing cells were shown to display both neuronal and glial phenotypes. We also delivered the temperature sensitive allele of SV40 Large-T antigen and obtained a high level of expression of the immortalizing oncoprotein in the cells. Colonies of Large-T antigen immunoreactive cells were indeed visible 10 days after transfection.

Original languageEnglish
Pages (from-to)161-166
Number of pages6
JournalMolecular Brain Research
Volume42
Issue number1
DOIs
Publication statusPublished - Nov 1996

Keywords

  • Human CNS precursor cell
  • Large-T antigen
  • Lipofectamine
  • Transfection

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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