Nonmucoid Pseudomonas aeruginosa expresses alginate in the lungs of patients with cystic fibrosis and in a mouse model

Alessandra Bragonzi, Dieter Worlitzsch, Gerald B. Pier, Petra Timpert, Martina Ulrich, Morten Hentzer, Jens Bo Andersen, Michael Givskov, Massimo Conese, Gerd Döring

Research output: Contribution to journalArticle

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Abstract

Background. In patients with cystic fibrosis (CF), lung infection with mucoid Pseudomonas aeruginosa strains overexpressing the exopolysaccaride alginate is preceded by colonization with nonmucoid strains. We investigated the kinetics, impact of environmental signals, and genetics of P. aeruginosa alginate expression in a mouse model and in patients with CF. Methods. Using indirect immunofluorescence, microarray technology and real-time reverse-transcription polymerase chain reaction, we assessed alginate gene expression during aerobic and anaerobic growth of the nonmucoid strain PAO1 in vitro, in a mouse lung-infection model and in sputum specimens from patients with CF infected with nonmucoid or mucoid P, aeruginosa strains. Results. Anaerobic conditions increased the transcription of alginate genes in vitro and in murine lungs within 24 h. Alginate production by PAO1 in murine lungs and by nonmucoid P. aeruginosa strains in patients with CF was reversible after in vitro culture under aerobic conditions. A subpopulation of P. aeruginosa clones revealing stable alginate production was detected in murine lungs 2 weeks after infection. Conclusions. Anaerobiosis and lung infection rapidly induce alginate production by gene regulation in nonmucoid P. aeruginosa. This trait may contribute to early persistence, leading to chronic P. aeruginosa infection once stable mucoid strains are generated.

Original languageEnglish
Pages (from-to)410-419
Number of pages10
JournalJournal of Infectious Diseases
Volume192
Issue number3
DOIs
Publication statusPublished - Aug 1 2005

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Cystic Fibrosis
Pseudomonas aeruginosa
Lung
Infection
Anaerobiosis
Pseudomonas Infections
Indirect Fluorescent Antibody Technique
alginic acid
Sputum
Genes
Reverse Transcription
Clone Cells
Technology
Gene Expression
Polymerase Chain Reaction
Growth
In Vitro Techniques

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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Nonmucoid Pseudomonas aeruginosa expresses alginate in the lungs of patients with cystic fibrosis and in a mouse model. / Bragonzi, Alessandra; Worlitzsch, Dieter; Pier, Gerald B.; Timpert, Petra; Ulrich, Martina; Hentzer, Morten; Andersen, Jens Bo; Givskov, Michael; Conese, Massimo; Döring, Gerd.

In: Journal of Infectious Diseases, Vol. 192, No. 3, 01.08.2005, p. 410-419.

Research output: Contribution to journalArticle

Bragonzi, A, Worlitzsch, D, Pier, GB, Timpert, P, Ulrich, M, Hentzer, M, Andersen, JB, Givskov, M, Conese, M & Döring, G 2005, 'Nonmucoid Pseudomonas aeruginosa expresses alginate in the lungs of patients with cystic fibrosis and in a mouse model', Journal of Infectious Diseases, vol. 192, no. 3, pp. 410-419. https://doi.org/10.1086/431516
Bragonzi, Alessandra ; Worlitzsch, Dieter ; Pier, Gerald B. ; Timpert, Petra ; Ulrich, Martina ; Hentzer, Morten ; Andersen, Jens Bo ; Givskov, Michael ; Conese, Massimo ; Döring, Gerd. / Nonmucoid Pseudomonas aeruginosa expresses alginate in the lungs of patients with cystic fibrosis and in a mouse model. In: Journal of Infectious Diseases. 2005 ; Vol. 192, No. 3. pp. 410-419.
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