Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus

M. Ligozzi, G. M. Rossolini, E. A. Tonin, R. Fontana

Research output: Contribution to journalArticle

Abstract

A DNA probe was developed by inserting, in the SmaI site of pBluescript sK, a 528-bp fragment of the gene responsible for intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus (mec determinant). The mec probe provided a useful tool for the rapid identification of the intrinsic resistance trait and for establishing guidelines for testing the in vitro susceptibility of S. aureus to beta-lactams.

Original languageEnglish
Pages (from-to)575-578
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume35
Issue number3
Publication statusPublished - 1991

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ASJC Scopus subject areas

  • Pharmacology (medical)

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