Normalne i granične vrijednosti mikronukleus-testa na limfocitima periferne krvi u ispitanika opće populacije Republike Hrvatske

Translated title of the contribution: Normal and cut-off values of the cytokinesis-block micronucleus assay on peripheral blood lymphocytes in the croatian general population

Nevenka Kopjar, Vilena Kašuba, Mirta Milić, Ružica Rozgaj, Davor Želježić, Goran Gajski, Marin Mladinić, Vera Garaj-Vrhovac

Research output: Contribution to journalArticle

Abstract

The cytokinesis-block micronucleus (CBMN) assay on peripheral blood lymphocytes is one of the most important methods employed in cytogenetic biomonitoring. For the purposes of biological dosimetry, it is important to know the spontaneous frequency of a biomarker and its normal values in general population. These values are used for population databases, which should be updated regularly. In this study, MN levels were investigated in cytokinesis-blocked lymphocytes of 200 healthy male and female blood donors selected at random from the general population of Croatia. The aim was to assess the variability and determine possible influences of external and/or internal factors on the background levels of MN and to establish the cut-off value for the CBMN assay. The background frequency of MN was (6.90±3.32) MN (median 7 MN) and the range was 0 to 18 MN per 1000 binuclear lymphocytes. The cut-off value, which corresponds to 95th percentile of the distribution of 200 individual values, was 12.5 MN. Spontaneous formation of MN was influenced by sex, age, and smoking. Women had higher MN levels than men. However, only age and smoking significantly increased the values of all parameters evaluated by the CBMN assay. Since the existing literature data on smoking-related formation of MN are contradictory, we will continue these investigations to resolve how the number of cigarettes smoked per day and the duration of smoking in years influence the results of the CBMN assay. Our results are consistent with the background MN frequencies reported by other cytogenetic laboratories worldwide. Normal and cut-off values estimated in this study will be used to update the current general population data and as reference for occupationally or accidental exposure.

Translated title of the contributionNormal and cut-off values of the cytokinesis-block micronucleus assay on peripheral blood lymphocytes in the croatian general population
Original languageUndefined/Unknown
Pages (from-to)219-234
Number of pages16
JournalArhiv za Higijenu Rada i Toksikologiju
Volume61
Issue number2
DOIs
Publication statusPublished - Jun 1 2010

Keywords

  • age
  • cytogenetic monitoring
  • gender
  • genome instability
  • risk
  • smoking habits
  • spontaneous formation of micronuclei

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Toxicology

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