COLTURE DI CHERATINOCITI UMANI NORMALI: PRINCIPALI MODELLI ED APPLICAZIONI CLINICHE

Translated title of the contribution: Normal human keratinocyte cultures: Main models and clinical applications

G. Zambruno, M. L. Santantonio, A. Giannetti

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In the last fifteen years various epidermal cell (EC) culture systems have been developed suitable both for investigation purposes and for clinical applications. In organ and explant cultures skin pieces are grown to obtain in the first case vertical proliferation of epidermis and in the second outward migration and proliferation of EC. In isolated EC cultures, EC suspensions are seeded on plastic or specific substrates (collagens, fibronectin, 3T3 fibroblasts). EC attach to the substrate, proliferate to form a confluent multilayered epithelium and can be subcultured. Using a serum-free medium (MCDB 153) EC can be grown in culture free of any undefined supplement. In organotypical culture systems, EC are seded on a biological matrix (dermis, dermal equivalent) and lifted at the air-liquid interface. Under these conditions a high level of differentiation of the cultured epidermis can be obtained. The availability of a culture-grown epithelium, similar to the in vivo epidermis, has led to the use of cultured epidermal sheets for the treatment of wounds, in particular burns. The therapeutic results have been quite promising.

Original languageItalian
Pages (from-to)59-77
Number of pages19
JournalGiornale Italiano di Dermatologia e Venereologia
Volume125
Issue number3
Publication statusPublished - 1990

Fingerprint

Keratinocytes
Epidermis
Epithelium
Cell Culture Techniques
Skin
Organ Culture Techniques
Serum-Free Culture Media
Dermis
Burns
Fibronectins
Plastics
Suspensions
Collagen
Fibroblasts
Air
Cell Proliferation
Wounds and Injuries
Therapeutics

ASJC Scopus subject areas

  • Dermatology

Cite this

COLTURE DI CHERATINOCITI UMANI NORMALI : PRINCIPALI MODELLI ED APPLICAZIONI CLINICHE. / Zambruno, G.; Santantonio, M. L.; Giannetti, A.

In: Giornale Italiano di Dermatologia e Venereologia, Vol. 125, No. 3, 1990, p. 59-77.

Research output: Contribution to journalArticle

@article{42405c5ce19f433982089802d80839ef,
title = "COLTURE DI CHERATINOCITI UMANI NORMALI: PRINCIPALI MODELLI ED APPLICAZIONI CLINICHE",
abstract = "In the last fifteen years various epidermal cell (EC) culture systems have been developed suitable both for investigation purposes and for clinical applications. In organ and explant cultures skin pieces are grown to obtain in the first case vertical proliferation of epidermis and in the second outward migration and proliferation of EC. In isolated EC cultures, EC suspensions are seeded on plastic or specific substrates (collagens, fibronectin, 3T3 fibroblasts). EC attach to the substrate, proliferate to form a confluent multilayered epithelium and can be subcultured. Using a serum-free medium (MCDB 153) EC can be grown in culture free of any undefined supplement. In organotypical culture systems, EC are seded on a biological matrix (dermis, dermal equivalent) and lifted at the air-liquid interface. Under these conditions a high level of differentiation of the cultured epidermis can be obtained. The availability of a culture-grown epithelium, similar to the in vivo epidermis, has led to the use of cultured epidermal sheets for the treatment of wounds, in particular burns. The therapeutic results have been quite promising.",
author = "G. Zambruno and Santantonio, {M. L.} and A. Giannetti",
year = "1990",
language = "Italian",
volume = "125",
pages = "59--77",
journal = "Minerva dermatologica",
issn = "0392-0488",
publisher = "Edizioni Minerva Medica S.p.A.",
number = "3",

}

TY - JOUR

T1 - COLTURE DI CHERATINOCITI UMANI NORMALI

T2 - PRINCIPALI MODELLI ED APPLICAZIONI CLINICHE

AU - Zambruno, G.

AU - Santantonio, M. L.

AU - Giannetti, A.

PY - 1990

Y1 - 1990

N2 - In the last fifteen years various epidermal cell (EC) culture systems have been developed suitable both for investigation purposes and for clinical applications. In organ and explant cultures skin pieces are grown to obtain in the first case vertical proliferation of epidermis and in the second outward migration and proliferation of EC. In isolated EC cultures, EC suspensions are seeded on plastic or specific substrates (collagens, fibronectin, 3T3 fibroblasts). EC attach to the substrate, proliferate to form a confluent multilayered epithelium and can be subcultured. Using a serum-free medium (MCDB 153) EC can be grown in culture free of any undefined supplement. In organotypical culture systems, EC are seded on a biological matrix (dermis, dermal equivalent) and lifted at the air-liquid interface. Under these conditions a high level of differentiation of the cultured epidermis can be obtained. The availability of a culture-grown epithelium, similar to the in vivo epidermis, has led to the use of cultured epidermal sheets for the treatment of wounds, in particular burns. The therapeutic results have been quite promising.

AB - In the last fifteen years various epidermal cell (EC) culture systems have been developed suitable both for investigation purposes and for clinical applications. In organ and explant cultures skin pieces are grown to obtain in the first case vertical proliferation of epidermis and in the second outward migration and proliferation of EC. In isolated EC cultures, EC suspensions are seeded on plastic or specific substrates (collagens, fibronectin, 3T3 fibroblasts). EC attach to the substrate, proliferate to form a confluent multilayered epithelium and can be subcultured. Using a serum-free medium (MCDB 153) EC can be grown in culture free of any undefined supplement. In organotypical culture systems, EC are seded on a biological matrix (dermis, dermal equivalent) and lifted at the air-liquid interface. Under these conditions a high level of differentiation of the cultured epidermis can be obtained. The availability of a culture-grown epithelium, similar to the in vivo epidermis, has led to the use of cultured epidermal sheets for the treatment of wounds, in particular burns. The therapeutic results have been quite promising.

UR - http://www.scopus.com/inward/record.url?scp=0025323782&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025323782&partnerID=8YFLogxK

M3 - Articolo

C2 - 2198230

AN - SCOPUS:0025323782

VL - 125

SP - 59

EP - 77

JO - Minerva dermatologica

JF - Minerva dermatologica

SN - 0392-0488

IS - 3

ER -