Abstract
The use of HPLC coupled on-line with a mass spectrometer is a very powerful tool in order to analyze intact PLs molecular species (PMS) without the need of derivatization, thus decreasing the risk of artifacts formation. A normal-phase HPLC-ESI-MS-MS method has been developed in order to study the human blood mononuclear cell PMS composition. This method was applied to characterize PMS from seven CF subjects and from seven age-matched healthy subjects. More than 140 phospholipid molecular species from phosphatidylethanolamine (PE), plasmalogen phosphatidylethanolamine (pPE), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylcholine (PC) and sphingomyelin (Sph) were identified and compared. Differences between the two groups were found in pPE (p16:0/22:6), pPE (p18:0/22:6), PE (16:0/20:4) and PC (16:0/18:2) which were significantly lower in CF subjects and in PC (16:0/16:1) which was significantly higher in CF subjects.
| Original language | English |
|---|---|
| Pages (from-to) | 173-186 |
| Number of pages | 14 |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 810 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Oct 25 2004 |
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Keywords
- Blood mononuclear cells
- Cystic fibrosis
- HPLC
- Mass spectrometry
- Phospholipids
ASJC Scopus subject areas
- Biochemistry
Cite this
Normal phase liquid chromatography-electrospray ionization tandem mass spectrometry analysis of phospholipid molecular species in blood mononuclear cells : Application to cystic fibrosis. / Malavolta, Marco; Bocci, Fabio; Boselli, Emanuele; Frega, Natale G.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 810, No. 2, 25.10.2004, p. 173-186.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Normal phase liquid chromatography-electrospray ionization tandem mass spectrometry analysis of phospholipid molecular species in blood mononuclear cells
T2 - Application to cystic fibrosis
AU - Malavolta, Marco
AU - Bocci, Fabio
AU - Boselli, Emanuele
AU - Frega, Natale G.
PY - 2004/10/25
Y1 - 2004/10/25
N2 - The use of HPLC coupled on-line with a mass spectrometer is a very powerful tool in order to analyze intact PLs molecular species (PMS) without the need of derivatization, thus decreasing the risk of artifacts formation. A normal-phase HPLC-ESI-MS-MS method has been developed in order to study the human blood mononuclear cell PMS composition. This method was applied to characterize PMS from seven CF subjects and from seven age-matched healthy subjects. More than 140 phospholipid molecular species from phosphatidylethanolamine (PE), plasmalogen phosphatidylethanolamine (pPE), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylcholine (PC) and sphingomyelin (Sph) were identified and compared. Differences between the two groups were found in pPE (p16:0/22:6), pPE (p18:0/22:6), PE (16:0/20:4) and PC (16:0/18:2) which were significantly lower in CF subjects and in PC (16:0/16:1) which was significantly higher in CF subjects.
AB - The use of HPLC coupled on-line with a mass spectrometer is a very powerful tool in order to analyze intact PLs molecular species (PMS) without the need of derivatization, thus decreasing the risk of artifacts formation. A normal-phase HPLC-ESI-MS-MS method has been developed in order to study the human blood mononuclear cell PMS composition. This method was applied to characterize PMS from seven CF subjects and from seven age-matched healthy subjects. More than 140 phospholipid molecular species from phosphatidylethanolamine (PE), plasmalogen phosphatidylethanolamine (pPE), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylcholine (PC) and sphingomyelin (Sph) were identified and compared. Differences between the two groups were found in pPE (p16:0/22:6), pPE (p18:0/22:6), PE (16:0/20:4) and PC (16:0/18:2) which were significantly lower in CF subjects and in PC (16:0/16:1) which was significantly higher in CF subjects.
KW - Blood mononuclear cells
KW - Cystic fibrosis
KW - HPLC
KW - Mass spectrometry
KW - Phospholipids
UR - http://www.scopus.com/inward/record.url?scp=4544222361&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4544222361&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2004.07.001
DO - 10.1016/j.jchromb.2004.07.001
M3 - Article
C2 - 15380713
AN - SCOPUS:4544222361
VL - 810
SP - 173
EP - 186
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 2
ER -