Normal phase liquid chromatography-electrospray ionization tandem mass spectrometry analysis of phospholipid molecular species in blood mononuclear cells: Application to cystic fibrosis

Marco Malavolta, Fabio Bocci, Emanuele Boselli, Natale G. Frega

Research output: Contribution to journal › Article › peer-review

Abstract

The use of HPLC coupled on-line with a mass spectrometer is a very powerful tool in order to analyze intact PLs molecular species (PMS) without the need of derivatization, thus decreasing the risk of artifacts formation. A normal-phase HPLC-ESI-MS-MS method has been developed in order to study the human blood mononuclear cell PMS composition. This method was applied to characterize PMS from seven CF subjects and from seven age-matched healthy subjects. More than 140 phospholipid molecular species from phosphatidylethanolamine (PE), plasmalogen phosphatidylethanolamine (pPE), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylcholine (PC) and sphingomyelin (Sph) were identified and compared. Differences between the two groups were found in pPE (p16:0/22:6), pPE (p18:0/22:6), PE (16:0/20:4) and PC (16:0/18:2) which were significantly lower in CF subjects and in PC (16:0/16:1) which was significantly higher in CF subjects.

Original languageEnglish
Pages (from-to)173-186
Number of pages14
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume810
Issue number2
DOIs
Publication statusPublished - Oct 25 2004

Keywords

  • Blood mononuclear cells
  • Cystic fibrosis
  • HPLC
  • Mass spectrometry
  • Phospholipids

ASJC Scopus subject areas

  • Biochemistry

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