Abstract
The micronucleus test in peripheral blood lymphocytes is the most widely validated technique to evaluate the DNA damage and chromosomal instability in human populations. The test is largely applied in monitoring environmental and occupational exposure to genotoxic agents. It was also proposed as a biomarker of risk/susceptibility for cancer and other degenerative diseases. The availability of “normal values” in healthy populations is a main requisite for the assay application in human biomonitoring. Age and gender-related ranges of micronucleated binucleated cells (MNBN) baseline values were established in a group of 103 healthy platelet donors (50 males and 53 females) not recently exposed to genotoxic agents and characterized for demographic, lifestyle and dietary factors. Repeatability of the test by the same scorer was evaluated. Reproducibility was estimated through analysis of repeated blood samples. High correlation between the results of the three blood samplings in two separate scoring sessions of MNBN/1000BN (R2 values were 0.83, 0.74 and 0.68; P < 0.0001) and PI values (R2 values were 0.69, 0.62 and 0.65; P < 0.0001) was detected. High consistency among the values obtained in three different samplings in the same individual was observed (Intraclass Correlation Coefficient (ICC) = 0.905, (95% CI = 0.868–0.933, P < 0.0001) The range of “normal” values predicted on the basis of the results of the present study appears to be sufficiently narrow to warrant application of the assay in the comparison of data obtained from groups of exposed or susceptible subjects, supporting its use in preventive programs. The large inter-individual variability predicted by the model used in the present study hampers a clinical application of the assay at individual level. The method applied in the present study represents a generally applicable model to derive “normal values” in any population, as an essential step before starting a biomonitoring study.
| Original language | English |
|---|---|
| Pages (from-to) | 513-522 |
| Number of pages | 10 |
| Journal | Science of the Total Environment |
| Volume | 652 |
| DOIs | |
| Publication status | Published - Feb 20 2019 |
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Keywords
- Biomonitoring
- Chromosomal instability
- Cytome assay
- Healthy subjects
- Micronucleus
- Occupational exposure
ASJC Scopus subject areas
- Environmental Engineering
- Environmental Chemistry
- Waste Management and Disposal
- Pollution
Cite this
“Normal values” for the lymphocyte cytokinesis-block micronucleus cytome parameters : Repeatability and reproducibility in a healthy reference population. / Bolognesi, Claudia; Bonelli, Luigina; Compalati, Andrea; Ferla, Valentina; Stagnaro, Laura; Ubezio, Gianluca; Bruzzi, Paolo.
In: Science of the Total Environment, Vol. 652, 20.02.2019, p. 513-522.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - “Normal values” for the lymphocyte cytokinesis-block micronucleus cytome parameters
T2 - Repeatability and reproducibility in a healthy reference population
AU - Bolognesi, Claudia
AU - Bonelli, Luigina
AU - Compalati, Andrea
AU - Ferla, Valentina
AU - Stagnaro, Laura
AU - Ubezio, Gianluca
AU - Bruzzi, Paolo
PY - 2019/2/20
Y1 - 2019/2/20
N2 - The micronucleus test in peripheral blood lymphocytes is the most widely validated technique to evaluate the DNA damage and chromosomal instability in human populations. The test is largely applied in monitoring environmental and occupational exposure to genotoxic agents. It was also proposed as a biomarker of risk/susceptibility for cancer and other degenerative diseases. The availability of “normal values” in healthy populations is a main requisite for the assay application in human biomonitoring. Age and gender-related ranges of micronucleated binucleated cells (MNBN) baseline values were established in a group of 103 healthy platelet donors (50 males and 53 females) not recently exposed to genotoxic agents and characterized for demographic, lifestyle and dietary factors. Repeatability of the test by the same scorer was evaluated. Reproducibility was estimated through analysis of repeated blood samples. High correlation between the results of the three blood samplings in two separate scoring sessions of MNBN/1000BN (R2 values were 0.83, 0.74 and 0.68; P < 0.0001) and PI values (R2 values were 0.69, 0.62 and 0.65; P < 0.0001) was detected. High consistency among the values obtained in three different samplings in the same individual was observed (Intraclass Correlation Coefficient (ICC) = 0.905, (95% CI = 0.868–0.933, P < 0.0001) The range of “normal” values predicted on the basis of the results of the present study appears to be sufficiently narrow to warrant application of the assay in the comparison of data obtained from groups of exposed or susceptible subjects, supporting its use in preventive programs. The large inter-individual variability predicted by the model used in the present study hampers a clinical application of the assay at individual level. The method applied in the present study represents a generally applicable model to derive “normal values” in any population, as an essential step before starting a biomonitoring study.
AB - The micronucleus test in peripheral blood lymphocytes is the most widely validated technique to evaluate the DNA damage and chromosomal instability in human populations. The test is largely applied in monitoring environmental and occupational exposure to genotoxic agents. It was also proposed as a biomarker of risk/susceptibility for cancer and other degenerative diseases. The availability of “normal values” in healthy populations is a main requisite for the assay application in human biomonitoring. Age and gender-related ranges of micronucleated binucleated cells (MNBN) baseline values were established in a group of 103 healthy platelet donors (50 males and 53 females) not recently exposed to genotoxic agents and characterized for demographic, lifestyle and dietary factors. Repeatability of the test by the same scorer was evaluated. Reproducibility was estimated through analysis of repeated blood samples. High correlation between the results of the three blood samplings in two separate scoring sessions of MNBN/1000BN (R2 values were 0.83, 0.74 and 0.68; P < 0.0001) and PI values (R2 values were 0.69, 0.62 and 0.65; P < 0.0001) was detected. High consistency among the values obtained in three different samplings in the same individual was observed (Intraclass Correlation Coefficient (ICC) = 0.905, (95% CI = 0.868–0.933, P < 0.0001) The range of “normal” values predicted on the basis of the results of the present study appears to be sufficiently narrow to warrant application of the assay in the comparison of data obtained from groups of exposed or susceptible subjects, supporting its use in preventive programs. The large inter-individual variability predicted by the model used in the present study hampers a clinical application of the assay at individual level. The method applied in the present study represents a generally applicable model to derive “normal values” in any population, as an essential step before starting a biomonitoring study.
KW - Biomonitoring
KW - Chromosomal instability
KW - Cytome assay
KW - Healthy subjects
KW - Micronucleus
KW - Occupational exposure
UR - http://www.scopus.com/inward/record.url?scp=85055263220&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85055263220&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2018.10.187
DO - 10.1016/j.scitotenv.2018.10.187
M3 - Article
VL - 652
SP - 513
EP - 522
JO - Science of the Total Environment
JF - Science of the Total Environment
SN - 0048-9697
ER -