Abstract
Aim: To describe the phenotypic and genotypic profiles of Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-Kp) strains isolated from patients with invasive infections at an Italian university hospital in order to assess the epidemiological trend. Methods: An observational prospective study was undertaken at the University Hospital of Sassari, Italy to detect KPC-Kp strains in patients with invasive bacteraemia. Isolates were identified phenotypically; carbapenemase production was assessed using phenotypic and genotypic methods. Sequencing of blaKPC genes, pulsed-field gel electrophoresis and multi-locus sequence typing were performed. Results: During the period 2015–2017, 46 cases of invasive infection with K. pneumoniae were recorded. Two-thirds (67.4%) of the patients were male, and the mean age was 69.4 years. Most patients had at least one comorbidity (56.5%) and/or had been hospitalized previously (70.5%), 81.8% had current or recent medical device use, and 85.4% had recent antibiotic exposure. The mortality rate was 52.3%. A multi-drug-resistant pattern (including carbapenems, fluoroquinolones, third-/fourth-generation cephalosporins) was shown for all K. pneumoniae isolates. KPC-3 and -2 were produced by all strains. The most common sequence types were 512 (91.3%) and 101 (8.7%), grouped into three clusters (A, A1 and B). Conclusions: A high incidence of KPC-Kp in patients with invasive infections was recorded at an Italian university hospital compared with the incidence measured before 2015. This study confirmed the importance of the KPC-3 carbapenemase variant, as reported by other Italian studies. High mortality and comorbidity rates appear to be associated with KPC-Kp infection.
| Original language | English |
|---|---|
| Pages (from-to) | 413-418 |
| Number of pages | 6 |
| Journal | Journal of Hospital Infection |
| Volume | 99 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Aug 1 2018 |
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Keywords
- Genotypic analysis
- Klebsiella pneumoniae carbapenemase-producing K. pneumoniae
- Surveillance
ASJC Scopus subject areas
- Microbiology (medical)
- Infectious Diseases
Cite this
Nosocomial transmission of carbapenem-resistant Klebsiella pneumoniae in an Italian university hospital : a molecular epidemiological study. / Sotgiu, G.; Are, B. M.; Pesapane, L.; Palmieri, A.; Muresu, N.; Cossu, A.; Dettori, M.; Azara, A.; Mura, I. I.; Cocuzza, C.; Aliberti, S.; Piana, A.
In: Journal of Hospital Infection, Vol. 99, No. 4, 01.08.2018, p. 413-418.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nosocomial transmission of carbapenem-resistant Klebsiella pneumoniae in an Italian university hospital
T2 - a molecular epidemiological study
AU - Sotgiu, G.
AU - Are, B. M.
AU - Pesapane, L.
AU - Palmieri, A.
AU - Muresu, N.
AU - Cossu, A.
AU - Dettori, M.
AU - Azara, A.
AU - Mura, I. I.
AU - Cocuzza, C.
AU - Aliberti, S.
AU - Piana, A.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Aim: To describe the phenotypic and genotypic profiles of Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-Kp) strains isolated from patients with invasive infections at an Italian university hospital in order to assess the epidemiological trend. Methods: An observational prospective study was undertaken at the University Hospital of Sassari, Italy to detect KPC-Kp strains in patients with invasive bacteraemia. Isolates were identified phenotypically; carbapenemase production was assessed using phenotypic and genotypic methods. Sequencing of blaKPC genes, pulsed-field gel electrophoresis and multi-locus sequence typing were performed. Results: During the period 2015–2017, 46 cases of invasive infection with K. pneumoniae were recorded. Two-thirds (67.4%) of the patients were male, and the mean age was 69.4 years. Most patients had at least one comorbidity (56.5%) and/or had been hospitalized previously (70.5%), 81.8% had current or recent medical device use, and 85.4% had recent antibiotic exposure. The mortality rate was 52.3%. A multi-drug-resistant pattern (including carbapenems, fluoroquinolones, third-/fourth-generation cephalosporins) was shown for all K. pneumoniae isolates. KPC-3 and -2 were produced by all strains. The most common sequence types were 512 (91.3%) and 101 (8.7%), grouped into three clusters (A, A1 and B). Conclusions: A high incidence of KPC-Kp in patients with invasive infections was recorded at an Italian university hospital compared with the incidence measured before 2015. This study confirmed the importance of the KPC-3 carbapenemase variant, as reported by other Italian studies. High mortality and comorbidity rates appear to be associated with KPC-Kp infection.
AB - Aim: To describe the phenotypic and genotypic profiles of Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-Kp) strains isolated from patients with invasive infections at an Italian university hospital in order to assess the epidemiological trend. Methods: An observational prospective study was undertaken at the University Hospital of Sassari, Italy to detect KPC-Kp strains in patients with invasive bacteraemia. Isolates were identified phenotypically; carbapenemase production was assessed using phenotypic and genotypic methods. Sequencing of blaKPC genes, pulsed-field gel electrophoresis and multi-locus sequence typing were performed. Results: During the period 2015–2017, 46 cases of invasive infection with K. pneumoniae were recorded. Two-thirds (67.4%) of the patients were male, and the mean age was 69.4 years. Most patients had at least one comorbidity (56.5%) and/or had been hospitalized previously (70.5%), 81.8% had current or recent medical device use, and 85.4% had recent antibiotic exposure. The mortality rate was 52.3%. A multi-drug-resistant pattern (including carbapenems, fluoroquinolones, third-/fourth-generation cephalosporins) was shown for all K. pneumoniae isolates. KPC-3 and -2 were produced by all strains. The most common sequence types were 512 (91.3%) and 101 (8.7%), grouped into three clusters (A, A1 and B). Conclusions: A high incidence of KPC-Kp in patients with invasive infections was recorded at an Italian university hospital compared with the incidence measured before 2015. This study confirmed the importance of the KPC-3 carbapenemase variant, as reported by other Italian studies. High mortality and comorbidity rates appear to be associated with KPC-Kp infection.
KW - Genotypic analysis
KW - Klebsiella pneumoniae carbapenemase-producing K. pneumoniae
KW - Surveillance
UR - http://www.scopus.com/inward/record.url?scp=85048720975&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85048720975&partnerID=8YFLogxK
U2 - 10.1016/j.jhin.2018.03.033
DO - 10.1016/j.jhin.2018.03.033
M3 - Article
AN - SCOPUS:85048720975
VL - 99
SP - 413
EP - 418
JO - Journal of Hospital Infection
JF - Journal of Hospital Infection
SN - 0195-6701
IS - 4
ER -