Abstract
The physico-chemical properties and the electrophoretic behavior of the novel set of monomers reported by (Simò-Alfonso et al., Electrophoresis 1996, 17, 723-731) have been evaluated. Of utmost importance was the combination of high hydrophilicity and extreme hydrolytic stability, most desired properties for any electrophoretic matrix, especially for protein fractionation. One of these monomers (N-acryloylaminopropanol, AAP) was found indeed to be extremely hydrophilic (with a partition coefficient P of only 0.10, vs. P = 0.13 for N-acryloylaminoethoxyethanol and P = 0.20 for acrylamide) and to possess excellent stability to alkaline hydrolysis. Its hydrolysis constant (0.008 L mol-1 min-1), as a free monomer, in an alkaline milieu, was found to be about one order of magnitude lower than conventional acrylamide (0.05 L mol-1 min-1). In the polymer state, the resistance to hydrolysis of poly(AAP) was assessed as 500 times greater than a conventional poly(acrylamide) matrix.
| Original language | English |
|---|---|
| Pages (from-to) | 732-737 |
| Number of pages | 6 |
| Journal | Electrophoresis |
| Volume | 17 |
| Issue number | 4 |
| Publication status | Published - Apr 1996 |
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Keywords
- N-Acryloylaminopropanol
- N-Substituted acrylamides
ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
Novel acrylamido monomers with higher hydrophilicity and improved hydrolytic stability : II. Properties of N-acryloylaminopropanol. / Simò-Alfonso, Ernesto; Gelfi, Cecilia; Sebastiano, Roberto; Citterio, Attilio; Righetti, Pier Giorgio.
In: Electrophoresis, Vol. 17, No. 4, 04.1996, p. 732-737.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Novel acrylamido monomers with higher hydrophilicity and improved hydrolytic stability
T2 - II. Properties of N-acryloylaminopropanol
AU - Simò-Alfonso, Ernesto
AU - Gelfi, Cecilia
AU - Sebastiano, Roberto
AU - Citterio, Attilio
AU - Righetti, Pier Giorgio
PY - 1996/4
Y1 - 1996/4
N2 - The physico-chemical properties and the electrophoretic behavior of the novel set of monomers reported by (Simò-Alfonso et al., Electrophoresis 1996, 17, 723-731) have been evaluated. Of utmost importance was the combination of high hydrophilicity and extreme hydrolytic stability, most desired properties for any electrophoretic matrix, especially for protein fractionation. One of these monomers (N-acryloylaminopropanol, AAP) was found indeed to be extremely hydrophilic (with a partition coefficient P of only 0.10, vs. P = 0.13 for N-acryloylaminoethoxyethanol and P = 0.20 for acrylamide) and to possess excellent stability to alkaline hydrolysis. Its hydrolysis constant (0.008 L mol-1 min-1), as a free monomer, in an alkaline milieu, was found to be about one order of magnitude lower than conventional acrylamide (0.05 L mol-1 min-1). In the polymer state, the resistance to hydrolysis of poly(AAP) was assessed as 500 times greater than a conventional poly(acrylamide) matrix.
AB - The physico-chemical properties and the electrophoretic behavior of the novel set of monomers reported by (Simò-Alfonso et al., Electrophoresis 1996, 17, 723-731) have been evaluated. Of utmost importance was the combination of high hydrophilicity and extreme hydrolytic stability, most desired properties for any electrophoretic matrix, especially for protein fractionation. One of these monomers (N-acryloylaminopropanol, AAP) was found indeed to be extremely hydrophilic (with a partition coefficient P of only 0.10, vs. P = 0.13 for N-acryloylaminoethoxyethanol and P = 0.20 for acrylamide) and to possess excellent stability to alkaline hydrolysis. Its hydrolysis constant (0.008 L mol-1 min-1), as a free monomer, in an alkaline milieu, was found to be about one order of magnitude lower than conventional acrylamide (0.05 L mol-1 min-1). In the polymer state, the resistance to hydrolysis of poly(AAP) was assessed as 500 times greater than a conventional poly(acrylamide) matrix.
KW - N-Acryloylaminopropanol
KW - N-Substituted acrylamides
UR - http://www.scopus.com/inward/record.url?scp=0029658953&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029658953&partnerID=8YFLogxK
M3 - Article
VL - 17
SP - 732
EP - 737
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 4
ER -