Novel approach for evaluation of Bacteroides fragilis protective role against Bartonella henselae liver damage in immunocompromised murine model

Chiara Pagliuca, Annunziata G. Cicatiello, Roberta Colicchio, Adelaide Greco, Raimondo Cerciello, Luigi Auletta, Sandra Albanese, Elena Scaglione, Caterina Pagliarulo, Gabiria Pastore, Gelsomina Mansueto, Arturo Brunetti, Bice Avallone, Paola Salvatore

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bartonella henselae is a gram-negative facultative intracellular bacterium and is the causative agent of cat-scratch disease. Our previous data have established that Bacteroides fragilis colonization is able to prevent B. henselae damages through the polysaccharide A (PSA) in an experimental murine model. In order to determine whether the PSA is essential for the protection against pathogenic effects of B. henselae in immunocompromised hosts, SCID mice were co-infected with B. fragilis wild type or its mutant B. fragilis ΔPSA and the effects of infection on murine tissues have been observed by High-Frequency Ultrasound (HFUS), histopathological examination, and Transmission Electron Microscopy (TEM). For the first time, echostructure, hepatic lobes length, vascular alterations, and indirect signs of hepatic dysfunctions, routinely used as signs of disease in humans, have been analyzed in an immunocompromised murine model. Our findings showed echostructural alterations in all infected mice compared with the Phosphate Buffer Solution (PBS) control group; further, those infected with B. henselae and co-infected with B. henselae/B. fragilis ΔPSA presented the major echostructural alterations. Half of the mice infected with B. henselae and all those co-infected with B. henselae/B. fragilis ΔPSA have showed an altered hepatic echogenicity compared with the renal cortex. The echogenicity score of co-infected mice with B. henselae/B. fragilis ΔPSA differed significantly compared with the PBS control group (p < 0.05). Moreover the inflammation score of the histopathological evaluation was fairly concordant with ultrasound findings. Ultrastructural analysis performed by TEM revealed no significant alterations in liver samples of SCID mice infected with B. fragilis wild type while those infected with B. fragilis ΔPSA showed the presence of collagen around the main vessels compared with the PBS control group. The liver samples of mice infected with B. henselae showed macro-areas rich in collagen, stellate cells, and histiocytic cells. Interestingly, our data demonstrated that immunocompromised SCID mice infected with B. henselae and co-infected with B. henselae/B. fragilis ΔPSA showed the most severe morpho-structural liver damage. In addition, these results suggests that the HFUS together with histopathological evaluation could be considered good imaging approach to evaluate hepatic alterations.

Original languageEnglish
Article number1750
JournalFrontiers in Microbiology
Volume7
Issue numberNOV
DOIs
Publication statusPublished - Nov 7 2016

Fingerprint

Bartonella henselae
Bacteroides fragilis
Polysaccharides
Liver
SCID Mice
Buffers
Phosphates
Transmission Electron Microscopy
Control Groups
Collagen
Cat-Scratch Disease
Immunocompromised Host
Blood Vessels

Keywords

  • Bartonella henselae. Bacteroides fragilis
  • HFUS
  • Histological examination
  • Imaging
  • PSA
  • SCID mice
  • TEM

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Novel approach for evaluation of Bacteroides fragilis protective role against Bartonella henselae liver damage in immunocompromised murine model. / Pagliuca, Chiara; Cicatiello, Annunziata G.; Colicchio, Roberta; Greco, Adelaide; Cerciello, Raimondo; Auletta, Luigi; Albanese, Sandra; Scaglione, Elena; Pagliarulo, Caterina; Pastore, Gabiria; Mansueto, Gelsomina; Brunetti, Arturo; Avallone, Bice; Salvatore, Paola.

In: Frontiers in Microbiology, Vol. 7, No. NOV, 1750, 07.11.2016.

Research output: Contribution to journalArticle

Pagliuca, C, Cicatiello, AG, Colicchio, R, Greco, A, Cerciello, R, Auletta, L, Albanese, S, Scaglione, E, Pagliarulo, C, Pastore, G, Mansueto, G, Brunetti, A, Avallone, B & Salvatore, P 2016, 'Novel approach for evaluation of Bacteroides fragilis protective role against Bartonella henselae liver damage in immunocompromised murine model', Frontiers in Microbiology, vol. 7, no. NOV, 1750. https://doi.org/10.3389/fmicb.2016.01750
Pagliuca, Chiara ; Cicatiello, Annunziata G. ; Colicchio, Roberta ; Greco, Adelaide ; Cerciello, Raimondo ; Auletta, Luigi ; Albanese, Sandra ; Scaglione, Elena ; Pagliarulo, Caterina ; Pastore, Gabiria ; Mansueto, Gelsomina ; Brunetti, Arturo ; Avallone, Bice ; Salvatore, Paola. / Novel approach for evaluation of Bacteroides fragilis protective role against Bartonella henselae liver damage in immunocompromised murine model. In: Frontiers in Microbiology. 2016 ; Vol. 7, No. NOV.
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AU - Cicatiello, Annunziata G.

AU - Colicchio, Roberta

AU - Greco, Adelaide

AU - Cerciello, Raimondo

AU - Auletta, Luigi

AU - Albanese, Sandra

AU - Scaglione, Elena

AU - Pagliarulo, Caterina

AU - Pastore, Gabiria

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N2 - Bartonella henselae is a gram-negative facultative intracellular bacterium and is the causative agent of cat-scratch disease. Our previous data have established that Bacteroides fragilis colonization is able to prevent B. henselae damages through the polysaccharide A (PSA) in an experimental murine model. In order to determine whether the PSA is essential for the protection against pathogenic effects of B. henselae in immunocompromised hosts, SCID mice were co-infected with B. fragilis wild type or its mutant B. fragilis ΔPSA and the effects of infection on murine tissues have been observed by High-Frequency Ultrasound (HFUS), histopathological examination, and Transmission Electron Microscopy (TEM). For the first time, echostructure, hepatic lobes length, vascular alterations, and indirect signs of hepatic dysfunctions, routinely used as signs of disease in humans, have been analyzed in an immunocompromised murine model. Our findings showed echostructural alterations in all infected mice compared with the Phosphate Buffer Solution (PBS) control group; further, those infected with B. henselae and co-infected with B. henselae/B. fragilis ΔPSA presented the major echostructural alterations. Half of the mice infected with B. henselae and all those co-infected with B. henselae/B. fragilis ΔPSA have showed an altered hepatic echogenicity compared with the renal cortex. The echogenicity score of co-infected mice with B. henselae/B. fragilis ΔPSA differed significantly compared with the PBS control group (p < 0.05). Moreover the inflammation score of the histopathological evaluation was fairly concordant with ultrasound findings. Ultrastructural analysis performed by TEM revealed no significant alterations in liver samples of SCID mice infected with B. fragilis wild type while those infected with B. fragilis ΔPSA showed the presence of collagen around the main vessels compared with the PBS control group. The liver samples of mice infected with B. henselae showed macro-areas rich in collagen, stellate cells, and histiocytic cells. Interestingly, our data demonstrated that immunocompromised SCID mice infected with B. henselae and co-infected with B. henselae/B. fragilis ΔPSA showed the most severe morpho-structural liver damage. In addition, these results suggests that the HFUS together with histopathological evaluation could be considered good imaging approach to evaluate hepatic alterations.

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