Novel associations among HLA-DQA1 and -DQB1 alleles, revealed by high-resolution sequence-based typing (SBT)

C. Pera, L. Delfino, A. Longo, M. P. Pistillo, G. B. Ferrara

Research output: Contribution to journalArticlepeer-review

Abstract

Althought it is a valuable tool for the identification of HLA alleles, sequence-based typing (SBT) presents difficulties when used to determine HLA-DQA1 and -DQB1 alleles. Specifically, some HLA-DQA1 alleles have a three-base deletion at codon 56 of exon 2 that interferes with the sequencing read. Moreover, the frequently used primers for HLA-DQB1 may co-amplify the HLA-DQB2 pseudogene. To overcome these problems, we amplified DQA1 exon 2 using five group-specific polymerase chain reactions (PCRs) which allowed separation of deleted from non-deleted DQA1 alleles. DQB1 exon 2 was amplified using two group-specific amplifications. To increase typing resolution, we also analyzed DQA1 exons 1, 3 and 4 and DQB1 exon 3 by PCR using sequence-specific primers (PCR-SSP) or SET analysis. Using this method we found some important associations between DQA1 and DQB1 alleles: DQA1*05011 and DQB1*0201, DQA1*0505 and DQB1*03011, DQA1*01021 and DQB1*06, DQA1*01022 and DQB1*0502.

Original languageEnglish
Pages (from-to)275-279
Number of pages5
JournalTissue Antigens
Volume55
Issue number3
DOIs
Publication statusPublished - 2000

Keywords

  • HLA-DQA1 and DQB1
  • Linkage disequilibrium
  • PCR-SBT
  • PCR-SSP

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Fingerprint Dive into the research topics of 'Novel associations among HLA-DQA1 and -DQB1 alleles, revealed by high-resolution sequence-based typing (SBT)'. Together they form a unique fingerprint.

Cite this