Novel autoantigens recognized by CSF IgG from Hashimoto's encephalitis revealed by a proteomic approach

Beatrice Gini, Lovato Laura, Cianti Riccardo, Cecotti Laura, Silvia Marconi, Elena Anghileri, Armini Alessandro, Moretto Giuseppe, Bini Luca, Ferracci Franco, Bonetti Bruno

Research output: Contribution to journalArticlepeer-review

Abstract

To identify the target of IgG autoimmune response in Hashimoto's encephalopathy (HE), we studied the binding of IgG present in serum and cerebro-spinal fluid (CSF) from six patients with HE and 15 controls to human central nervous system (CNS) white matter antigens by 2D-PAGE and immunoblotting and by immunohistochemistry. We found that CSF IgG from HE patients specifically recognized 3 spots, which were identified as dimethylargininase-I (DDAHI) and aldehyde reductase-I (AKRIAI). DDAHI was present in two isoforms recognized respectively by five and four HE patients; immunohistochemistry with anti-DDAHI antiserum depicted endothelial cells in normal human CNS. AKRIAI was recognized by three HE CSF and this enzyme was widely distributed on neurons and endothelia by immunohistochemistry. IgG from HE CSF immunostained both neuronal and endothelial cells in mouse CNS. The presence of these autoantibodies selectively in the CSF of HE patients may have important diagnostic and pathogenetic implications, since the autoimmune response to these enzymes may lead to vascular and/or neuronal damage, two major mechanisms involved in the pathogenesis of HE.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalJournal of Neuroimmunology
Volume196
Issue number1-2
DOIs
Publication statusPublished - May 30 2008

Keywords

  • AKRIAI
  • Autoantibodies
  • DDAHI
  • Hashimoto's encephalitis
  • Proteomics

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

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