Novel Chinese hamster ultraviolet-sensitive mutants for excision repair form complementation groups 9 and 10

Miria Stefanini, Andrew R. Collins, Roberta Riboni, Maria Klaude, Elena Botta, David L. Mitchell, Fiorella Nuzzo

Research output: Contribution to journalArticle

Abstract

In this paper we demonstrate that the mutants CHO7PV and CHO4PV isolated by us from the CHO-K1 prol- cell line represent two new complementation groups of UV-sensitive excision repair-defective rodent mutants. We have classified the mutant CHO7PV as representative of Group 9 and CHO4PV as representative of Group 10. Cellular and biochemical characterization of these mutants indicates that they are moderately sensitive to a broad spectrum of mutagens (UV and mono-and bifunctional alkylating agents), partially unable to perform UV-induced DNA repair synthesis, and partially defective in the incision step of the DNA excision repair pathway and in the removal of the two main lesions caused by UV [cyclobutane pyrimidine dimers and (6-4) photoproducts]. In terms of UV survival and incision, CHO4PV is apparently more defective than CHO7PV (40% and 50% of wild-type survival, respectively, and 55% and 75% of wild-type incision), whereas when repair DNA synthesis and lesion removal are compared, CHO7PV seems to be more severely affected (30% of wild-type unscheduled DNA synthesis in CHO7PV and 60% in CHO4PV). This suggests a subtlety in the relation between removal of these specific lesions and overall repair capacity and survival.

Original languageEnglish
Pages (from-to)3965-3971
Number of pages7
JournalCancer Research
Volume51
Issue number15
Publication statusPublished - Aug 1 1991

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Stefanini, M., Collins, A. R., Riboni, R., Klaude, M., Botta, E., Mitchell, D. L., & Nuzzo, F. (1991). Novel Chinese hamster ultraviolet-sensitive mutants for excision repair form complementation groups 9 and 10. Cancer Research, 51(15), 3965-3971.