Novel evidence of PLC δ2 involvement in the regulation of the differential evolution of human aneurysms

M. Marchisio, G. M. Sabatino, A. Albanese, E. Santavenere, R. Buonaguidi, Sebastiano Miscia

Research output: Contribution to journalArticle

Abstract

The biological and molecular mechanisms which are responsible for the formation and possible evolution of human aneurysms are unknown. Previous investigations have pointed to the possible involvement of inositol specific-phospholipase C (PLC) in the mechanisms related to the formation or evolution of intracranial aneurysms, but, thus far, a relationship of one or more PLC isoforms with the biological signals influencing the fate of this lesion has not been demonstrated. The aim of this study was to investigate the expression, activity and possible modification of PLC isoforms in intracranial aneurysms in patients undergoing elective surgical repair after casual identification of unruptured aneurysms, or during emergency surgical repair of ruptured aneurysms. PLC and proliferating cell nuclear antigen (PCNA) expressions were detected by immunoistochemical analysis; PLC activity was obtained by measuring its hydrolytic activity on labelled PIP2; PKC activity was measured by total kinase activity assay. Results indicated no substantial differences between controls and aneurysms, with the only exception being PLC δ2 which was nearly absent in controls and ruptured aneurysms, while strongly expressed and functionally active in almost all unruptured aneurysms. In addition, its expression always correlated with the proliferation cell marker PCNA, while its specific activity always correlated to PKC activity. PLC δ2 distribution, regulation and role in human tissues are still unknown Therefore, although preliminary, these data provide a novel insight into the signalling machinery influencing the aneurismal progression.

Original languageEnglish
Pages (from-to)381-388
Number of pages8
JournalInternational Journal of Immunopathology and Pharmacology
Volume17
Issue number3
Publication statusPublished - Sep 2004

Fingerprint

Type C Phospholipases
Aneurysm
Ruptured Aneurysm
Proliferating Cell Nuclear Antigen
Intracranial Aneurysm
Protein Isoforms
Inositol
Emergencies
Phosphotransferases
Cell Proliferation

Keywords

  • Aneurysms
  • Phospholipase C
  • Protein-kinase C

ASJC Scopus subject areas

  • Pharmacology

Cite this

Novel evidence of PLC δ2 involvement in the regulation of the differential evolution of human aneurysms. / Marchisio, M.; Sabatino, G. M.; Albanese, A.; Santavenere, E.; Buonaguidi, R.; Miscia, Sebastiano.

In: International Journal of Immunopathology and Pharmacology, Vol. 17, No. 3, 09.2004, p. 381-388.

Research output: Contribution to journalArticle

Marchisio, M. ; Sabatino, G. M. ; Albanese, A. ; Santavenere, E. ; Buonaguidi, R. ; Miscia, Sebastiano. / Novel evidence of PLC δ2 involvement in the regulation of the differential evolution of human aneurysms. In: International Journal of Immunopathology and Pharmacology. 2004 ; Vol. 17, No. 3. pp. 381-388.
@article{a7972c3a6a7d47aeadb91d2a9dd7ed3d,
title = "Novel evidence of PLC δ2 involvement in the regulation of the differential evolution of human aneurysms",
abstract = "The biological and molecular mechanisms which are responsible for the formation and possible evolution of human aneurysms are unknown. Previous investigations have pointed to the possible involvement of inositol specific-phospholipase C (PLC) in the mechanisms related to the formation or evolution of intracranial aneurysms, but, thus far, a relationship of one or more PLC isoforms with the biological signals influencing the fate of this lesion has not been demonstrated. The aim of this study was to investigate the expression, activity and possible modification of PLC isoforms in intracranial aneurysms in patients undergoing elective surgical repair after casual identification of unruptured aneurysms, or during emergency surgical repair of ruptured aneurysms. PLC and proliferating cell nuclear antigen (PCNA) expressions were detected by immunoistochemical analysis; PLC activity was obtained by measuring its hydrolytic activity on labelled PIP2; PKC activity was measured by total kinase activity assay. Results indicated no substantial differences between controls and aneurysms, with the only exception being PLC δ2 which was nearly absent in controls and ruptured aneurysms, while strongly expressed and functionally active in almost all unruptured aneurysms. In addition, its expression always correlated with the proliferation cell marker PCNA, while its specific activity always correlated to PKC activity. PLC δ2 distribution, regulation and role in human tissues are still unknown Therefore, although preliminary, these data provide a novel insight into the signalling machinery influencing the aneurismal progression.",
keywords = "Aneurysms, Phospholipase C, Protein-kinase C",
author = "M. Marchisio and Sabatino, {G. M.} and A. Albanese and E. Santavenere and R. Buonaguidi and Sebastiano Miscia",
year = "2004",
month = "9",
language = "English",
volume = "17",
pages = "381--388",
journal = "International Journal of Immunopathology and Pharmacology",
issn = "0394-6320",
publisher = "Biomedical Research Press s.a.s.",
number = "3",

}

TY - JOUR

T1 - Novel evidence of PLC δ2 involvement in the regulation of the differential evolution of human aneurysms

AU - Marchisio, M.

AU - Sabatino, G. M.

AU - Albanese, A.

AU - Santavenere, E.

AU - Buonaguidi, R.

AU - Miscia, Sebastiano

PY - 2004/9

Y1 - 2004/9

N2 - The biological and molecular mechanisms which are responsible for the formation and possible evolution of human aneurysms are unknown. Previous investigations have pointed to the possible involvement of inositol specific-phospholipase C (PLC) in the mechanisms related to the formation or evolution of intracranial aneurysms, but, thus far, a relationship of one or more PLC isoforms with the biological signals influencing the fate of this lesion has not been demonstrated. The aim of this study was to investigate the expression, activity and possible modification of PLC isoforms in intracranial aneurysms in patients undergoing elective surgical repair after casual identification of unruptured aneurysms, or during emergency surgical repair of ruptured aneurysms. PLC and proliferating cell nuclear antigen (PCNA) expressions were detected by immunoistochemical analysis; PLC activity was obtained by measuring its hydrolytic activity on labelled PIP2; PKC activity was measured by total kinase activity assay. Results indicated no substantial differences between controls and aneurysms, with the only exception being PLC δ2 which was nearly absent in controls and ruptured aneurysms, while strongly expressed and functionally active in almost all unruptured aneurysms. In addition, its expression always correlated with the proliferation cell marker PCNA, while its specific activity always correlated to PKC activity. PLC δ2 distribution, regulation and role in human tissues are still unknown Therefore, although preliminary, these data provide a novel insight into the signalling machinery influencing the aneurismal progression.

AB - The biological and molecular mechanisms which are responsible for the formation and possible evolution of human aneurysms are unknown. Previous investigations have pointed to the possible involvement of inositol specific-phospholipase C (PLC) in the mechanisms related to the formation or evolution of intracranial aneurysms, but, thus far, a relationship of one or more PLC isoforms with the biological signals influencing the fate of this lesion has not been demonstrated. The aim of this study was to investigate the expression, activity and possible modification of PLC isoforms in intracranial aneurysms in patients undergoing elective surgical repair after casual identification of unruptured aneurysms, or during emergency surgical repair of ruptured aneurysms. PLC and proliferating cell nuclear antigen (PCNA) expressions were detected by immunoistochemical analysis; PLC activity was obtained by measuring its hydrolytic activity on labelled PIP2; PKC activity was measured by total kinase activity assay. Results indicated no substantial differences between controls and aneurysms, with the only exception being PLC δ2 which was nearly absent in controls and ruptured aneurysms, while strongly expressed and functionally active in almost all unruptured aneurysms. In addition, its expression always correlated with the proliferation cell marker PCNA, while its specific activity always correlated to PKC activity. PLC δ2 distribution, regulation and role in human tissues are still unknown Therefore, although preliminary, these data provide a novel insight into the signalling machinery influencing the aneurismal progression.

KW - Aneurysms

KW - Phospholipase C

KW - Protein-kinase C

UR - http://www.scopus.com/inward/record.url?scp=8344220486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8344220486&partnerID=8YFLogxK

M3 - Article

C2 - 15461872

AN - SCOPUS:8344220486

VL - 17

SP - 381

EP - 388

JO - International Journal of Immunopathology and Pharmacology

JF - International Journal of Immunopathology and Pharmacology

SN - 0394-6320

IS - 3

ER -