Novel image processing methods for the analysis of calcium dynamics in glial cells

Andrea Fanelli, Jasmine Ion Titapiccolo, Federico Esposti, Maddalena Ripamonti, Antonio Malgaroli, Maria G. Signorini

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Calcium (Ca 2+) waves and Ca 2+ oscillations within cells initiate a wide range of physiological processes including control of cell signaling, gene expression, secretion, and cell migration. A thorough analysis of Ca 2+ waves in glial cells provides information not only about the subcellular location of signaling processing events but also about nonneuronal or intercellular signaling pathways, their timing, routes, spatial domains, and coordination. In this study, three novel image processing methods have been applied to the study of Ca 2+ dynamics in cells. These bring additional information to the methods already available in the literature, providing insight into the analysis of calcium dynamics in fluorescence recordings and defining bidimensional maps that give a complete and detailed description of calcium intracellular behavior. The application of these processing methods to glial cells highlighted the complex 2-D Ca 2+ dynamics phenomena, the location of calcium uptake and release microdomains on the endoplasmic reticulum, and the correlation between different calcium signals inside the cell. A perinuclear zone acting as a filter and regulator of intracellular calcium waves was detected: it acts as a controller of calcium fluxes between the cytoplasm and the nucleus.

Original languageEnglish
Article number5928390
Pages (from-to)2640-2647
Number of pages8
JournalIEEE Transactions on Biomedical Engineering
Volume58
Issue number9
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Calcium
Image processing
Cell signaling
Processing
Neuroglia
Gene expression
Process control
Fluorescence
Fluxes
Controllers

Keywords

  • Biomedical image processing
  • calcium signaling
  • cell signaling
  • molecular biology
  • signal processing

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Fanelli, A., Titapiccolo, J. I., Esposti, F., Ripamonti, M., Malgaroli, A., & Signorini, M. G. (2011). Novel image processing methods for the analysis of calcium dynamics in glial cells. IEEE Transactions on Biomedical Engineering, 58(9), 2640-2647. [5928390]. https://doi.org/10.1109/TBME.2011.2160344

Novel image processing methods for the analysis of calcium dynamics in glial cells. / Fanelli, Andrea; Titapiccolo, Jasmine Ion; Esposti, Federico; Ripamonti, Maddalena; Malgaroli, Antonio; Signorini, Maria G.

In: IEEE Transactions on Biomedical Engineering, Vol. 58, No. 9, 5928390, 09.2011, p. 2640-2647.

Research output: Contribution to journalArticle

Fanelli, A, Titapiccolo, JI, Esposti, F, Ripamonti, M, Malgaroli, A & Signorini, MG 2011, 'Novel image processing methods for the analysis of calcium dynamics in glial cells', IEEE Transactions on Biomedical Engineering, vol. 58, no. 9, 5928390, pp. 2640-2647. https://doi.org/10.1109/TBME.2011.2160344
Fanelli, Andrea ; Titapiccolo, Jasmine Ion ; Esposti, Federico ; Ripamonti, Maddalena ; Malgaroli, Antonio ; Signorini, Maria G. / Novel image processing methods for the analysis of calcium dynamics in glial cells. In: IEEE Transactions on Biomedical Engineering. 2011 ; Vol. 58, No. 9. pp. 2640-2647.
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