Novel inducers of BECN1-independent autophagy: Cis-unsaturated fatty acids

Mireia Niso-Santano, José Manuel Bravo San Pedro, Maria Chiara Maiuri, Nektarios Tavernarakis, Francesco Cecconi, Frank Madeo, Patrice Codogno, Lorenzo Galluzzi, Guido Kroemer

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The induction of autophagy usually requires the activation of PIK3C3/ VPS34 (phosphatidylinositol 3-kinase, catalytic subunit type 3) within a multiprotein complex that contains BECN1 (Beclin 1, autophagy related). PIK3C3 catalyzes the conversion of phosphatidylinositol into phosphatidylinositol 3-phosphate (PtdIns3P). PtdIns3P associates with growing phagophores, which recruit components of the autophagic machinery, including the lipidated form of MAP1LC3B/LC3 (microtubule-associated protein 1 light chain 3 b). Depletion of BECN1, PIK3C3 or some of their interactors suppresses the formation of MAP1LC3B+ phagophores or autophagosomes elicited by most physiological stimuli, including saturated fatty acids. We observed that cis-unsaturated fatty acids stimulate the generation of cytosolic puncta containing lipidated MAP1LC3B as well as the autophagic turnover of long-lived proteins in the absence of PtdIns3P accumulation. In line with this notion, cis-unsaturated fatty acids require neither BECN1 nor PIK3C3 to stimulate the autophagic flux. Such a BECN1-independent autophagic response is phylogenetically conserved, manifesting in yeast, nematodes, mice and human cells. Importantly, MAP1LC3B+ puncta elicited by cisunsaturated fatty acids colocalize with Golgi apparatus markers. Moreover, the structural and functional collapse of the Golgi apparatus induced by brefeldin A inhibits cis-unsaturated fatty acid-triggered autophagy. It is tempting to speculate that the well-established healthpromoting effects of cis-unsaturated fatty acids are linked to their unusual capacity to stimulate noncanonical, BECN1-independent autophagic responses.

Original languageEnglish
Pages (from-to)575-577
Number of pages3
JournalAutophagy
Volume11
Issue number3
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Autophagy
Unsaturated Fatty Acids
Golgi Apparatus
Class III Phosphatidylinositol 3-Kinases
Fatty Acids
Brefeldin A
Multiprotein Complexes
Microtubule-Associated Proteins
Phosphatidylinositols
Yeasts
Light
phosphatidylinositol 3-phosphate
Proteins

Keywords

  • Caenorhabditis elegans
  • Noncanonical autophagy
  • Oleate
  • Palmitate
  • Saccharomyces cerevisiae
  • Stearate

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Niso-Santano, M., Pedro, J. M. B. S., Maiuri, M. C., Tavernarakis, N., Cecconi, F., Madeo, F., ... Kroemer, G. (2015). Novel inducers of BECN1-independent autophagy: Cis-unsaturated fatty acids. Autophagy, 11(3), 575-577. https://doi.org/10.1080/15548627.2015.1017222

Novel inducers of BECN1-independent autophagy : Cis-unsaturated fatty acids. / Niso-Santano, Mireia; Pedro, José Manuel Bravo San; Maiuri, Maria Chiara; Tavernarakis, Nektarios; Cecconi, Francesco; Madeo, Frank; Codogno, Patrice; Galluzzi, Lorenzo; Kroemer, Guido.

In: Autophagy, Vol. 11, No. 3, 01.01.2015, p. 575-577.

Research output: Contribution to journalArticle

Niso-Santano, M, Pedro, JMBS, Maiuri, MC, Tavernarakis, N, Cecconi, F, Madeo, F, Codogno, P, Galluzzi, L & Kroemer, G 2015, 'Novel inducers of BECN1-independent autophagy: Cis-unsaturated fatty acids', Autophagy, vol. 11, no. 3, pp. 575-577. https://doi.org/10.1080/15548627.2015.1017222
Niso-Santano M, Pedro JMBS, Maiuri MC, Tavernarakis N, Cecconi F, Madeo F et al. Novel inducers of BECN1-independent autophagy: Cis-unsaturated fatty acids. Autophagy. 2015 Jan 1;11(3):575-577. https://doi.org/10.1080/15548627.2015.1017222
Niso-Santano, Mireia ; Pedro, José Manuel Bravo San ; Maiuri, Maria Chiara ; Tavernarakis, Nektarios ; Cecconi, Francesco ; Madeo, Frank ; Codogno, Patrice ; Galluzzi, Lorenzo ; Kroemer, Guido. / Novel inducers of BECN1-independent autophagy : Cis-unsaturated fatty acids. In: Autophagy. 2015 ; Vol. 11, No. 3. pp. 575-577.
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