Novel insight into stem cell trafficking in dystrophic muscles

Andrea Farini, Chiara Villa, Adrian Manescu, Fabrizio Fiori, Alessandra Giuliani, Paola Razini, Clementina Sitzia, Giulia D. Del Fraro, Marzia Belicchi, Mirella Meregalli, Franco Rustichelli, Yvan Torrente

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Recently published reports have described possible cellular therapy approaches to regenerate muscle tissues using arterial route delivery. However, the kinetic of distribution of these migratory stem cells within injected animal muscular dystrophy models is unknown. Using living X-ray computed microtomography, we established that intra-arterially injected stem cells traffic to multiple muscle tissues for several hours until their migration within dystrophic muscles. Injected stem cells express multiple traffic molecules, including VLA-4, LFA-1, CD44, and the chemokine receptor CXCR4, which are likely to direct these cells into dystrophic muscles. In fact, the majority of intra-arterially injected stem cells access the muscle tissues not immediately after the injection, but after several rounds of recirculation. We set up a new, living, 3D-imaging approach, which appears to be an important way to investigate the kinetic of distribution of systemically injected stem cells within dystrophic muscle tissues, thereby providing supportive data for future clinical applications.

Original languageEnglish
Pages (from-to)3059-3067
Number of pages9
JournalInternational Journal of Nanomedicine
Volume7
DOIs
Publication statusPublished - 2012

Fingerprint

Stem cells
Muscle
Stem Cells
Muscles
Tissue
Animal Muscular Dystrophy
Integrin alpha4beta1
X-Ray Microtomography
Lymphocyte Function-Associated Antigen-1
Kinetics
Chemokine Receptors
Animals
Imaging techniques
X rays
Injections
Molecules

Keywords

  • CD133 stem cells
  • Dystrophic muscles
  • Iron nanoparticles
  • Micro-CT

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

Cite this

Novel insight into stem cell trafficking in dystrophic muscles. / Farini, Andrea; Villa, Chiara; Manescu, Adrian; Fiori, Fabrizio; Giuliani, Alessandra; Razini, Paola; Sitzia, Clementina; Del Fraro, Giulia D.; Belicchi, Marzia; Meregalli, Mirella; Rustichelli, Franco; Torrente, Yvan.

In: International Journal of Nanomedicine, Vol. 7, 2012, p. 3059-3067.

Research output: Contribution to journalArticle

Farini, A, Villa, C, Manescu, A, Fiori, F, Giuliani, A, Razini, P, Sitzia, C, Del Fraro, GD, Belicchi, M, Meregalli, M, Rustichelli, F & Torrente, Y 2012, 'Novel insight into stem cell trafficking in dystrophic muscles', International Journal of Nanomedicine, vol. 7, pp. 3059-3067. https://doi.org/10.2147/IJN.S30595
Farini A, Villa C, Manescu A, Fiori F, Giuliani A, Razini P et al. Novel insight into stem cell trafficking in dystrophic muscles. International Journal of Nanomedicine. 2012;7:3059-3067. https://doi.org/10.2147/IJN.S30595
Farini, Andrea ; Villa, Chiara ; Manescu, Adrian ; Fiori, Fabrizio ; Giuliani, Alessandra ; Razini, Paola ; Sitzia, Clementina ; Del Fraro, Giulia D. ; Belicchi, Marzia ; Meregalli, Mirella ; Rustichelli, Franco ; Torrente, Yvan. / Novel insight into stem cell trafficking in dystrophic muscles. In: International Journal of Nanomedicine. 2012 ; Vol. 7. pp. 3059-3067.
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