Novel markers of normal and neoplastic human plasmacytoid dendritic cells

Teresa Marafioti, Jennifer C. Paterson, Erica Ballabio, Kaaren K. Reichard, Sara Tedoldi, Kevin Hollowood, Michael Dictor, Martin Leo Hansmann, Stefano A. Pileri, Martin J. Dyer, Silvano Sozzani, Ivan Dikic, Andrey S. Shaw, Tony Petrella, Harald Stein, Peter G. Isaacson, Fabio Facchetti, David Y. Mason

Research output: Contribution to journalArticle

Abstract

Plasmacyloid dendritic cells (pDCs) are involved in innate immunity (eg, by secreting interferons) and also give rise to CD4+CD56+ hematodermic neoplasms. We report extensive characterization of human pDCs in routine tissue samples, documenting the expression of 19 immunohistologic markers, including signaling molecules (eg, BLNK), transcription factors (eg, ICSBP/IRF8 and PU.1), and Toll-like receptors (TLR7, TLR9). Many of these molecules are expressed in other cell types (principally B cells), but the adaptor protein CD2AP was essentially restricted to pDCs, and is therefore a novel immunohistologic marker for use in tissue biopsies. We found little evidence for activation-associated morphologic or phenotypic changes in conditions where pDCs are greatly increased (eg, Kikuchi disease). Most of the molecules were retained in the majority of pDC neoplasms, and 3 (BCL11A, CD2AP, and ICSBP/IRF8) were also commonly negative in leukemia cutis (acute myeloid leukemia in the skin), a tumor that may mimic pDC neoplasia. In summary, we have documented a range of molecules (notably those associated with B cells) expressed by pDCs in tissues and peripheral blood (where pDCs were detectable in cytospins at a frequency of <1% of mononuclear cells) and also defined potential new markers (in particular CD2AP) for the diagnosis of pDC tumors.

Original languageEnglish
Pages (from-to)3778-3792
Number of pages15
JournalBlood
Volume111
Issue number7
DOIs
Publication statusPublished - Apr 1 2008

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ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Marafioti, T., Paterson, J. C., Ballabio, E., Reichard, K. K., Tedoldi, S., Hollowood, K., Dictor, M., Hansmann, M. L., Pileri, S. A., Dyer, M. J., Sozzani, S., Dikic, I., Shaw, A. S., Petrella, T., Stein, H., Isaacson, P. G., Facchetti, F., & Mason, D. Y. (2008). Novel markers of normal and neoplastic human plasmacytoid dendritic cells. Blood, 111(7), 3778-3792. https://doi.org/10.1182/blood-2007-10-117531