Novel medicinal mushroom blend as a promising supplement in integrative oncology: A multi-tiered study using 4t1 triple-negative mouse breast cancer model

E. Roda, F. De Luca, C. Di Iorio, D. Ratto, S. Siciliani, B. Ferrari, F. Cobelli, G. Borsci, E.C. Priori, S. Chinosi, A. Ronchi, R. Franco, R. Di Francia, M. Berretta, C.A. Locatelli, A. Gregori, E. Savino, M.G. Bottone, P. Rossi

Research output: Contribution to journalArticlepeer-review

Abstract

Although medicinal mushroom extracts have been proposed as promising anti-cancer agents, their precise impacts on metastatic breast cancer are still to be clarified. For this purpose, the present study exploited the effect of a novel medicinal mushroom blend, namely Micotherapy U-care, in a 4T1 triple-negative mouse breast cancer model. Mice were orally administered with Micotherapy U-care, consisting of a mixture of Agaricus blazei, Ophiocordyceps sinensis, Ganoderma lucidum, Grifola frondosa, and Lentinula edodes. The syngeneic tumor-bearing mice were generated by injecting 4T1 cells in both supplemented and non-supplemented mice. After sacrifice 25 days later, specific endpoints and pathological outcomes of the murine pulmonary tissue were evaluated. (i) Histopathological and ultrastructural analysis and (ii) immunohistochemical assessment of TGF-ß1, IL-6 and NOS2, COX2, SOD1 as markers of inflammation and oxidative stress were performed. The QoL was comparatively evaluated. Micotherapy U-care supplementation, starting before 4T1 injection and lasting until the end of the experiment, dramatically reduced the pulmonary metastases density, also triggering a decrease of fibrotic response, and reducing IL-6, NOS, and COX2 expression. SOD1 and TGF-ß1 results were also discussed. These findings support the valuable potential of Micotherapy U-care as adjuvant therapy in the critical management of triple-negative breast cancer. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Original languageEnglish
Article number3479
JournalInternational Journal of Molecular Sciences
Volume21
Issue number10
DOIs
Publication statusPublished - 2020

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